亲水性类弹性蛋白多肽的温度触发相分离
Temperature-triggered phase separation of a hydrophilic resilin-like polypeptide.
作者信息
Li Linqing, Luo Tianzhi, Kiick Kristi L
机构信息
Department of Materials Science and Engineering, University of Delaware, Newark, Delaware, 19716, USA.
出版信息
Macromol Rapid Commun. 2015 Jan;36(1):90-5. doi: 10.1002/marc.201400521. Epub 2014 Nov 26.
Abstract
Temperature-triggered phase separation of recombinant proteins has offered substantial opportunities in the design of nanoparticles for a variety of applications. Herein, the temperature-triggered phase separation behavior of a recombinant hydrophilic resilin-like polypeptide (RLP) is described. The transition temperature and sizes of RLP-based nanoparticles can be modulated based on variations in polypeptide concentration, salt identity, ionic strength, pH, and denaturing agents, as indicated via UV-Vis spectroscopy and dynamic light scattering (DLS). The irreversible particle formation is coupled with secondary conformational changes from a random coil conformation to a more ordered β-sheet structure. These RLP-based nanoparticles could find potential use as mechanically-responsive components in drug delivery, nanospring, nanotransducer, and biosensor applications.
摘要
重组蛋白的温度触发相分离为设计用于各种应用的纳米颗粒提供了大量机会。在此描述了一种重组亲水性类弹性蛋白多肽（RLP）的温度触发相分离行为。通过紫外可见光谱和动态光散射（DLS）表明，基于RLP的纳米颗粒的转变温度和尺寸可根据多肽浓度、盐种类、离子强度、pH值和变性剂的变化进行调节。不可逆的颗粒形成与从无规卷曲构象到更有序的β-折叠结构的二级构象变化相关联。这些基于RLP的纳米颗粒在药物递送、纳米弹簧、纳米换能器和生物传感器应用中作为机械响应组件具有潜在用途。
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