The purposes of this study included examining the reliability of reactive strength index-modified (RSImod), the relationships between RSImod and force-time variables, and the differences in RSImod between male and female collegiate athletes. One hundred six Division I collegiate athletes performed unloaded and loaded countermovement jumps (CMJs). Intraclass correlation coefficients and typical error expressed as a coefficient of variation were used to establish the relative and absolute reliability of RSImod, respectively. Pearson zero-order product-moment correlation coefficients were used to examine the relationships between RSImod and rate of force development, peak force (PF), and peak power (PP) during unloaded and loaded jumping conditions. Finally, independent samples t-tests were used to examine the sex differences in RSImod between male and female athletes. Intraclass correlation coefficient values for RSImod ranged from 0.96 to 0.98, and typical error values ranged from 7.5 to 9.3% during all jumping conditions. Statistically significant correlations existed between RSImod and all force-time variables examined for male and female athletes during both jumping conditions (p ≤ 0.05). Statistically significant differences in RSImod existed between male and female athletes during both unloaded and loaded CMJs (p < 0.001). Reactive strength index-modified seems to be a reliable performance measurement in male and female athletes. Reactive strength index-modified may be described and used as a measure of explosiveness. Stronger relationships between RSImod, PF, and PP existed in female athletes as compared with that in male athletes; however, further evidence investigating these relationships is needed before conclusive statements can be made. Male athletes produced greater RSImod values as compared with that produced by female athletes.