Umeda Makoto, Tsuno Akiko, Okagami Yoshihide, Tsuchiya Fumito, Izumi Yuichi, Ishikawa Isao
Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan Division of Oral and Maxillo Facial Surgery, National Center for Global Health and Medicine, Tokyo, Japan Department of Research and Development, J. Morita Manufacturing Corporation, Kyoto, Japan Department of Pharmacy, Tokyo Medical and Dental University Hospital, Faculty of Dentistry, Tokyo, Japan International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Tokyo, Japan Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
J Investig Clin Dent. 2011 Nov;2(4):268-74. doi: 10.1111/j.2041-1626.2011.00071.x. Epub 2011 Jul 7.
Light-emitting diodes have been investigated as new light activators for photodynamic therapy. We investigated the bactericidal effects of high-power, red light-emitting diodes on two periodontopathic bacteria in vitro.
A light-emitting diode (intensity: 1100 mW/cm(2) , peak wavelength: 650 nm) was used to irradiate a bacterial solution for either 10 or 20 s. Bacterial solutions (Porphyromonas gingivalis or Aggregatibacter actinomycetemcomitans) at a concentration of 2.5 × 10(6) c.f.u./mL were mixed with an equal volume of either methylene blue or toluidine blue O (0-20 μg/mL) and added to titer plate wells. The plate wells were irradiated with red light-emitting diode light from a distance of 22 or 40 mm. The contents were diluted, and 50 μL was smeared onto blood agar plates. After 1 week of culturing, bacterial c.f.u. were counted.
The light-emitting diode energy density was estimated to be approximately 4 and 8 J/cm(2) after 10 and 20 s of irradiation, respectively. Red light-emitting diode irradiation for 10 s from a distance of 22 mm, combined with methylene blue at concentrations >10 μg/mL, completely killed Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans.
High-power, red light-emitting diode irradiation with a low concentration of dye showed effective bactericidal effects against two periodontopathic bacteria.
发光二极管已被作为光动力疗法的新型光激活剂进行研究。我们在体外研究了高功率红色发光二极管对两种牙周病原菌的杀菌作用。
使用发光二极管（强度：1100 mW/cm²，峰值波长：650 nm）对细菌溶液照射10秒或20秒。将浓度为2.5×10⁶ c.f.u./mL的细菌溶液（牙龈卟啉单胞菌或伴放线聚集杆菌）与等体积的亚甲蓝或甲苯胺蓝O（0 - 20 μg/mL）混合，并添加到滴定板孔中。从22或40 mm的距离用红色发光二极管光照射板孔。将内容物稀释，取50 μL涂抹在血琼脂平板上。培养1周后，计算细菌菌落形成单位。
照射10秒和20秒后，发光二极管的能量密度估计分别约为4和8 J/cm²。从22 mm的距离进行10秒的红色发光二极管照射，与浓度>10 μg/mL的亚甲蓝联合使用，可完全杀死牙龈卟啉单胞菌和伴放线聚集杆菌。
低浓度染料的高功率红色发光二极管照射对两种牙周病原菌显示出有效的杀菌作用。
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