Chair of Periodontology and Oral Medicine, Faculty of Medicine, Jagiellonian University Medical College, Montelupich 4, 31-155, Kraków, Poland.
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Kraków, Poland.
Photodiagnosis Photodyn Ther. 2017 Dec;20:28-34. doi: 10.1016/j.pdpdt.2017.08.008. Epub 2017 Aug 18.
The main goal of periodontal therapy is to eliminate the infection spreading in periodontium. Antimicrobial photodynamic therapy may be applied in order to eradicate pathogens remaining in periodontal tissues after conventional mechanical debridement, to improve the treatment results. The aim of this in vitro study was to evaluate the susceptibility of selected key periopathogens to toluidine blue O-mediated photodynamic inactivation and the influence of photosensitizer's concentration and light dose on the effectiveness of this process.
Following bacterial strains were used in the experiments: Porphyromonas gingivalis ATCC 33277, Aggregatibacter actinomyctemecomitans ATCC 33384, Fusobacterium nucleatum ATCC 10953. Toluidine blue O (TBO) was used in concentration ranging from 0.004 to 0.5mg/mL. Irradiation was performed by a non-laser red light source.
Complete eradication of P. gingivalis was obtained upon the application of TBO in the concentration of 0.1mg/mL and the highest light dose. A, actinomycetemcomitans was, in turn, not susceptible to photodynamic inactivation regardless of the dosimetric parameters applied. High viability reductions were also obtained for F. nucleatum, however no complete eradication. The effectiveness of photodynamic inactivation of susceptible periopathogens was dependent on the light dose and photosensitizer's concentration.
Periopathogens differ in terms of their susceptibility to photodynamic inactivation. Antimicrobial PDT may be valuable in the treatment of those cases of periodontal disease, in which P. gingivalis is a dominating pathogen. Microbiological examination prior to clinical application of aPDT may be recommended.
牙周病治疗的主要目标是消除牙周组织中的感染。为了消除常规机械清创后仍残留在牙周组织中的病原体，提高治疗效果，可以应用抗菌光动力疗法。本体外研究的目的是评估选定的主要牙周病原体对甲苯胺蓝 O 介导的光动力失活的敏感性，以及光敏剂浓度和光剂量对该过程效果的影响。
实验中使用了以下细菌菌株：牙龈卟啉单胞菌 ATCC 33277、伴放线放线杆菌 ATCC 33384、核梭杆菌 ATCC 10953。使用浓度范围为 0.004 至 0.5mg/mL 的甲苯胺蓝 O（TBO）。照射由非激光红光光源进行。
当应用浓度为 0.1mg/mL 的 TBO 和最高光剂量时，完全消除了 P. gingivalis。相反，无论应用何种剂量参数，A. actinomycetemcomitans 均不受光动力失活的影响。F. nucleatum 的存活能力也大大降低，但未能完全消除。光敏剂浓度和光剂量决定了易感牙周病原体光动力失活的效果。
牙周病原体对光动力失活的敏感性存在差异。抗菌光动力疗法可能对以 P. gingivalis 为主导病原体的牙周病治疗具有重要价值。在临床应用 aPDT 之前，建议进行微生物学检查。
