衰老和乙酰左旋肉碱对大鼠脑区能量代谢和胆碱能代谢的影响。

Effect of aging and acetyl-L-carnitine on energetic and cholinergic metabolism in rat brain regions.

作者信息

Curti D, Dagani F, Galmozzi M R, Marzatico F

机构信息

Institute of Pharmacology, University of Pavia, Italy.

出版信息

Mech Ageing Dev. 1989 Jan;47(1):39-45. doi: 10.1016/0047-6374(89)90005-5.

DOI:10.1016/0047-6374(89)90005-5

PMID:2542702

Abstract

The effect of aging and subchronic treatment with acetyl-L-carnitine (50 mg/kg per day) was studied on mitochondrial bioenergetics and cholinergic metabolism in non-synaptic mitochondria and synaptosomes isolated from cerebral cortex, hippocampus and striatum of rats aged 4, 11 and 18 months. Respiratory activity and cytochrome oxidase specific activity were unaffected by aging in non-synaptic mitochondria. In synaptosomes, pyruvate dehydrogenase, choline acetyltransferase and acetylcholinesterase specific activity remained unchanged, but the high-affinity choline uptake decreased in cerebral cortex and striatum of 18-month-old rats. Acetyl-L-carnitine treatment increased the high-affinity choline uptake in cerebral cortex of 18-month-old rats. The treatment caused also an increase in cytochrome oxidase activity in all the three cerebral regions and in choline uptake in the hippocampus, parameters that were not directly affected by aging processes.

摘要

研究了衰老以及用乙酰-L-肉碱（每天50毫克/千克）进行亚慢性治疗对从4、11和18月龄大鼠的大脑皮层、海马体和纹状体分离出的非突触线粒体和突触体中线粒体生物能量学和胆碱能代谢的影响。在非突触线粒体中，呼吸活性和细胞色素氧化酶比活性不受衰老影响。在突触体中，丙酮酸脱氢酶、胆碱乙酰转移酶和乙酰胆碱酯酶比活性保持不变，但18月龄大鼠大脑皮层和纹状体中的高亲和力胆碱摄取减少。乙酰-L-肉碱治疗增加了18月龄大鼠大脑皮层中的高亲和力胆碱摄取。该治疗还使所有三个脑区的细胞色素氧化酶活性以及海马体中的胆碱摄取增加，这些参数不受衰老过程的直接影响。