Ralston E, Hall Z W
Department of Physiology, School of Medicine, University of California, San Francisco 94143-0444.
Science. 1989 Jun 2;244(4908):1066-9. doi: 10.1126/science.2543074.
Specialized regions of muscle fibers may result from differential gene expression within a single fiber. In order to investigate the range of action of individual nuclei in multinucleated myotubes, C2 myoblasts were transfected to obtain stable cell lines that express a reporter protein that is targeted to the nucleus. Hybrid myotubes were then formed containing one or a few transfected nuclei as well as a large number of nuclei from the parental strain. In order to determine how far the products of a single nucleus extend, transfected nuclei were labeled with [3H]thymidine before fusion and the myotubes were stained to identify the reporter protein. In such myotubes the fusion protein was not confined to its nucleus of origin, but was restricted to nearby nuclei.
肌纤维的特化区域可能源于单个纤维内的差异基因表达。为了研究多核肌管中单个细胞核的作用范围，对C2成肌细胞进行转染，以获得稳定表达靶向细胞核的报告蛋白的细胞系。然后形成含有一个或几个转染细胞核以及大量亲本菌株细胞核的杂交肌管。为了确定单个细胞核的产物扩散范围，在融合前用[3H]胸腺嘧啶核苷标记转染的细胞核，并对肌管进行染色以鉴定报告蛋白。在这类肌管中，融合蛋白并不局限于其起源的细胞核，而是局限于附近的细胞核。
