Ralston E, Hall Z W
Department of Physiology, School of Medicine, University of California, San Francisco 94143-0444.
J Cell Biol. 1992 Dec;119(5):1063-8. doi: 10.1083/jcb.119.5.1063.
Although the proteins encoded by a single nucleus in multinucleated myotubes have a wide range of distributions within the myofiber, little is known about the distributions of their mRNAs. We have used hybrid myotubes in which one or a few nuclei are derived from myoblasts that express nonmuscle proteins to investigate this question. We find that three different mRNAs, encoding proteins that are, respectively, nuclear, cytoplasmic, and targeted to the ER, have similar distributions within myotubes. Each is confined to an area within approximately 100 microns of the nucleus that expresses it.
尽管多核肌管中单个细胞核所编码的蛋白质在肌纤维内具有广泛的分布，但对其mRNA的分布却知之甚少。我们利用杂种肌管来研究这个问题，其中一个或几个细胞核来源于表达非肌肉蛋白的成肌细胞。我们发现，三种不同的mRNA，分别编码核蛋白、细胞质蛋白和靶向内质网的蛋白，在肌管内具有相似的分布。每种mRNA都局限于表达它的细胞核周围约100微米的区域内。
