[使用多克隆和单克隆抗体通过免疫酶法分离人及猿猴甲型肝炎病毒的研究]

[Study of human and simian hepatitis A virus isolated by an immunoenzyme method using polyclonal and monoclonal antibodies].

作者信息

Karetnyĭ Iu V, Andzhaparidze A G, Orlova T M, Balaian M S

出版信息

Vopr Virusol. 1989 Jan-Feb;34(1):50-3.

Abstract

Hepatitis A viruses (HAV) isolated from man and Old World monkeys varied in their interaction with monoclonal antibodies (MCA) in enzyme immunoassay. All human HAV isolates reacted with the MCA under study (HIC19, H7C27, and K3-4C8), whereas the viruses isolated directly from monkeys did not react with H7C27 MCA and some of them also with H1C19 MCA. Simian HAV strains became capable of reacting with H7C27 MCA and H1C19 MCA after adaptation to cell culture. In tests with human and simian immune sera (polyclonal antibody to HAV) a number of human isolates interacted with human serum antibodies much more intensively and with simian serum antibodies.

摘要

从人和旧世界猴分离出的甲型肝炎病毒（HAV）在酶免疫测定中与单克隆抗体（MCA）的相互作用有所不同。所有人类HAV分离株都与所研究的MCA（HIC19、H7C27和K3-4C8）发生反应，而直接从猴子分离出的病毒不与H7C27 MCA反应，其中一些也不与H1C19 MCA反应。猿猴HAV毒株在适应细胞培养后能够与H7C27 MCA和H1C19 MCA发生反应。在用人和猿猴免疫血清（抗HAV多克隆抗体）进行的试验中，一些人类分离株与人类血清抗体的相互作用比与猿猴血清抗体的相互作用更为强烈。