用单克隆抗体通过固相放射免疫测定法和酶联免疫吸附测定法检测甲型肝炎病毒及抗体。
Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.
作者信息
Coulepis A G, Veale M F, MacGregor A, Kornitschuk M, Gust I D
出版信息
J Clin Microbiol. 1985 Jul;22(1):119-24. doi: 10.1128/jcm.22.1.119-124.1985.
Abstract
Monoclonal antibodies (K3-2F2 and K3-4C8) raised against hepatitis A virus were used to develop a solid-phase radioimmunoassay and enzyme-linked immunosorbent assay for the detection of hepatitis A virus and antibody. Assays with this pair of monoclonal antibodies were compared in parallel with similarly constructed solid-phase radioimmunoassays and enzyme-linked immunosorbent assays in which human polyclonal serum was used. The monoclonal antibody assay proved to be more sensitive for the detection of hepatitis A virus from fecal specimens as well as for anti-hepatitis A virus immunoglobulin G (IgG) and IgM in sera.
摘要
针对甲型肝炎病毒产生的单克隆抗体（K3-2F2和K3-4C8）被用于开发一种固相放射免疫测定法和酶联免疫吸附测定法，以检测甲型肝炎病毒和抗体。将使用这对单克隆抗体的测定法与使用人多克隆血清构建的类似固相放射免疫测定法和酶联免疫吸附测定法进行平行比较。事实证明，单克隆抗体测定法在检测粪便标本中的甲型肝炎病毒以及血清中的抗甲型肝炎病毒免疫球蛋白G（IgG）和IgM方面更为灵敏。
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