Modrzakowski M C, Dosch-Meier D, Hodinka R L
Can J Microbiol. 1983 Oct;29(10):1339-43. doi: 10.1139/m83-208.
Granule contents from rat polymorphonuclear neutrophils were prepared by extraction with 0.2 M acetate buffer (pH 4.0), dialyzed against phosphate-buffered saline (pH 7.0), and tested for bactericidal activity against selected target bacteria. Salmonella typhimurium LT-2 and a series of progressively rough lipopolysaccharide outer membrane mutants derived from it were used to monitor antimicrobial activity. Although an antimicrobial potential was present in rat granule contents for S. typhimurium, the growth of Pseudomonas aeruginosa PAO-1 in antimicrobial assay mixtures containing rat granule contents was substantially enhanced over control values. The growth enhancement property of the granule protein was heat resistant and promoted increased oxygen consumption by whole cells.
通过用0.2M乙酸盐缓冲液（pH 4.0）提取制备大鼠多形核中性粒细胞的颗粒内容物，对其进行磷酸盐缓冲盐水（pH 7.0）透析，并测试其对选定靶细菌的杀菌活性。鼠伤寒沙门氏菌LT-2及其衍生的一系列逐渐粗糙的脂多糖外膜突变体用于监测抗菌活性。尽管大鼠颗粒内容物对鼠伤寒沙门氏菌具有抗菌潜力，但在含有大鼠颗粒内容物的抗菌测定混合物中，铜绿假单胞菌PAO-1的生长比对照值显著增强。颗粒蛋白的生长促进特性具有耐热性，并促进全细胞耗氧量增加。
