Simon A, Mettenleiter T C, Rziha H J
Federal Research Centre for Virus Diseases of Animals, Tübingen, F.R.G.
J Gen Virol. 1989 May;70 ( Pt 5):1239-46. doi: 10.1099/0022-1317-70-5-1239.
The coding region of the glycoprotein complex gII of pseudorabies virus (PRV) is located in the unique long part of the genome on SalI subfragments 1A and 1G of BamHI fragment 1 (map units 0.105 to 0.130). Fragment 1G which includes the 3' end of the gII gene displays a size heterogeneity among different PRV strains and also within plaque isolates of a given strain. To reveal the cause of this heterogeneity and whether it might affect the gII-coding region we sequenced different 1G fragments of the PRV strains Ka, Phylaxia and Dessau, and determined the 3' end of the gII mRNA by S1 analysis. These data show that the size heterogeneity is caused by the presence of a variable number of tandemly repeated DNA sequence downstream but adjacent to the coding region of the glycoprotein gII gene. The 3' end of the gII mRNA was mapped about 24 bp upstream of the first repeat unit. A 15 bp sequence 5' GGGACGGAGGGGAGA 3' is repeated from three to over 50 times in different 1G fragments. It is the only repeat unit present in strain Ka, whereas the Phylaxia and Dessau strains show additional modifications.
伪狂犬病病毒（PRV）糖蛋白复合体gII的编码区位于基因组独特长区域内，在BamHI片段1的SalI亚片段1A和1G上（图谱单位0.105至0.130）。包含gII基因3'端的片段1G在不同PRV毒株之间以及同一毒株的蚀斑分离株中均表现出大小异质性。为了揭示这种异质性的原因以及它是否可能影响gII编码区，我们对PRV毒株Ka、Phylaxia和Dessau的不同1G片段进行了测序，并通过S1分析确定了gII mRNA的3'端。这些数据表明，大小异质性是由糖蛋白gII基因编码区下游但与之相邻的可变数量串联重复DNA序列的存在引起的。gII mRNA的3'端定位在第一个重复单元上游约24 bp处。在不同的1G片段中，15 bp序列5' GGGACGGAGGGGAGA 3'重复出现3至50多次。它是毒株Ka中唯一存在的重复单元，而Phylaxia和Dessau毒株则表现出额外的修饰。
