Ishikawa K, Tsutsui M, Taguchi K, Saitoh A, Muramatsu M
National Veterinary Assay Laboratory, Tokyo, Japan.
Vet Microbiol. 1996 Apr;49(3-4):267-72. doi: 10.1016/0378-1135(95)00182-4.
We have determined the nucleotide sequences of the major glycoprotein C (gC) gene of 3 pseudorabies virus (PRV) strains isolated in Japan, the USA, and Northern Ireland after gene amplification mediated by a polymerase chain reaction (PCR). The nucleotide and deduced amino acid sequence homologies among the strains were > 98% and > 97%, respectively. The restriction patterns of the amplified DNA fragments generated by restriction endonucleases SalI and SmaI revealed three genomic variations among the 15 PRV strains. The Japanese PRV isolates have identical restriction fragment patterns and differ from those of the non-Japanese isolates examined. Among 3 PRV strains with distinctive genotype each other, there is no significant difference in pathogenicity for the ddY mouse.
我们通过聚合酶链反应（PCR）介导的基因扩增，确定了在日本、美国和北爱尔兰分离的3株伪狂犬病病毒（PRV）主要糖蛋白C（gC）基因的核苷酸序列。这些毒株之间的核苷酸和推导氨基酸序列同源性分别>98%和>97%。限制性内切酶SalI和SmaI产生的扩增DNA片段的限制性图谱显示，在15株PRV毒株中有3种基因组变异。日本的PRV分离株具有相同的限制性片段模式，与所检测的非日本分离株不同。在3株彼此具有独特基因型的PRV毒株中，对ddY小鼠的致病性没有显著差异。
