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Requirement of A-A-U-A-A-A and adjacent downstream sequences for SV40 early polyadenylation.

作者信息

Kessler M M, Beckendorf R C, Westhafer M A, Nordstrom J L

出版信息

Nucleic Acids Res. 1986 Jun 25;14(12):4939-52. doi: 10.1093/nar/14.12.4939.

DOI:10.1093/nar/14.12.4939
PMID:3014439
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311502/
Abstract

RNA mapping experiments and chloramphenicol acetyltransferase assays were used to analyze polyadenylation in COS cells of transcripts from derivatives of pSV2-neo and pSV2-cat, in which the SV40 early poly(A) signal has been modified. Neither the sequence A-A-U-A-A-A nor the sequences located immediately downstream from it in the SV40 early gene appear to function by themselves as a poly(A) signal. When combined, however, these two elements form a poly(A) signal whose efficiency and specificity closely resemble those of the wild type signal. The addition of six nucleotides between the A-A-U-A-A-A sequence and the poly(A) site has no detectable effect on the efficiency or site of polyadenylation. Deletion of the 60 nucleotides immediately upstream from the hexanucleotide also has no detectable effect on polyadenylation. Therefore, A-A-U-A-A-A and sequences downstream from it appear to be sufficient for SV40 early polyadenylation.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ff6/311502/dfbe7781cba3/nar00281-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ff6/311502/c61213427b87/nar00281-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ff6/311502/31251faa9f76/nar00281-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ff6/311502/dfbe7781cba3/nar00281-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ff6/311502/c61213427b87/nar00281-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ff6/311502/31251faa9f76/nar00281-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ff6/311502/dfbe7781cba3/nar00281-0252-a.jpg

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Sequence homologies among the three yolk polypeptide (Yp) genes in Drosophila melanogaster.黑腹果蝇中三个卵黄多肽（Yp）基因之间的序列同源性。
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Analysis of adenovirus type 2 L1 RNA 3'-end formation in vivo and in vitro.2型腺病毒L1 RNA 3'末端在体内和体外形成的分析
J Virol. 1988 Apr;62(4):1464-8. doi: 10.1128/JVI.62.4.1464-1468.1988.
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10
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