Kruzel M L, Seman G, Georgiades J A
Amarillo Cell Culture Co. Inc. 79109.
Arch Immunol Ther Exp (Warsz). 1993;41(3-4):179-83.
Human interferon alpha (HuIFN-alpha) preparations induced in human peripheral white blood cells by Sendai virus were analyzed for activity after ultrafiltration, ion exchange, metal ion chromatography and isoelectrofocusing. It was found that HuIFN-alpha forms complexes with various physical properties and activities. Some of the IFN-alpha copurify with high molecular weight complexes. However, the low molecular weight fraction represent bulk of IFN activity. In isoelectrofocusing experiments, the former corresponds to IFN-alpha which possesses high pI (pH regions 8.5-10.5). An additional form of IFN-alpha are not complexed and possess a pI of 5.5-7.0. Our nIFN-alpha preparation preserve many of natural IFN isospecies and may have higher therapeutic value than the recombinant IFN-alpha preparations.
对仙台病毒诱导人外周血白细胞产生的人干扰素α（HuIFN-α）制剂进行超滤、离子交换、金属离子色谱和等电聚焦后分析其活性。发现HuIFN-α形成具有各种物理性质和活性的复合物。一些IFN-α与高分子量复合物共纯化。然而，低分子量部分代表了大部分的IFN活性。在等电聚焦实验中，前者对应于具有高pI（pH范围8.5 - 10.5）的IFN-α。另一种形式的IFN-α未形成复合物，pI为5.5 - 7.0。我们的天然IFN-α制剂保留了许多天然的IFN异构体，可能比重组IFN-α制剂具有更高的治疗价值。
