Pan Linjiang, Huang Suning, He Rongquan, Rong Minhua, Dang Yiwu, Chen Gang
Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region, 530021, PR China.
Department of Medical Oncology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region, 530021, PR China.
Eur J Med Res. 2014 Dec 2;19(1):68. doi: 10.1186/s40001-014-0068-2.
Aberrant expression of microRNA-148a (miR-148a) has been reported in several types of malignancies. However, its expression and clinicopathological significance in hepatocellular carcinoma (HCC) has not been entirely clarified. Our objective was to investigate the clinicopathological contribution of the miR-148a expression in HCC formalin-fixed paraffin-embedded (FFPE) tissues.
Eighty-nine HCC and their para-cancerous liver tissues were recruited. Total mRNA including miRNA was isolated and miR-148a expression was determined by using real time RT-qPCR. Furthermore, the relationship between the miR-148a level and clinicopathological features was explored.
Significantly lower miR-148a expression in HCC tissues was observed than that in adjacent noncancerous hepatic tissues. miR-148a expression was also correlated to clinical TNM stage, metastasis, status of capsular infiltration and numbers of tumor nodes.
Underexpression of miR-148a might be associated with HCC tumorigenesis and deterioration of HCC. miR-148a might act as a suppressor miRNA of HCC and it therefore has a potential role in prognosis of HCC patients.
已有报道称，微小RNA-148a（miR-148a）在多种恶性肿瘤中存在异常表达。然而，其在肝细胞癌（HCC）中的表达及临床病理意义尚未完全阐明。我们的目的是研究miR-148a在肝癌福尔马林固定石蜡包埋（FFPE）组织中的表达对临床病理的影响。
收集89例肝癌及其癌旁肝组织。分离包括miRNA在内的总mRNA，采用实时RT-qPCR法检测miR-148a的表达。此外，探讨miR-148a水平与临床病理特征之间的关系。
与相邻的非癌肝组织相比，肝癌组织中miR-148a的表达显著降低。miR-148a的表达还与临床TNM分期、转移、包膜浸润情况和肿瘤结节数量相关。
miR-148a表达不足可能与肝癌的发生及病情恶化有关。miR-148a可能作为肝癌的抑制性miRNA，因此在肝癌患者的预后中具有潜在作用。
