Talbot N C, Pursel V G, Rexroad C E, Caperna T J, Powell A M, Stone R T
U.S. Department of Agriculture, Beltsville Agricultural Research Center, Maryland 20705.
In Vitro Cell Dev Biol Anim. 1994 Dec;30A(12):851-8. doi: 10.1007/BF02639395.
The secondary culture of non-transformed parenchymal hepatocytes has not been possible. STO feeder cell-dependent secondary cultures of fetal pig hepatocytes were established by colony isolation from primary cultures of 26-d fetal livers. The liver cells had the typical polygonal morphology of parenchymal hepatocytes. They also spontaneously differentiated to form small biliary canaliculi between individual cells or progressed further to large multicellular duct-like structures or cells undergoing gross lipid accumulation and secretion. The secondary hepatocyte cultures expressed alpha-fetoprotein (AFP), albumin, and beta-fibrinogen mRNA, and conditioned medium from the cells contained elevated levels of transferrin and albumin. STO feeder cell co-culture may be useful for the sustainable culture of hepatocytes from other species.
未转化的实质肝细胞的传代培养一直无法实现。通过从26日龄胎肝原代培养物中进行集落分离，建立了依赖STO饲养层细胞的胎猪肝细胞传代培养体系。这些肝细胞具有实质肝细胞典型的多边形形态。它们还能自发分化，在单个细胞之间形成小的胆小管，或进一步发展为大型多细胞导管样结构，或出现大量脂质积累和分泌的细胞。传代肝细胞培养物表达甲胎蛋白（AFP）、白蛋白和β-纤维蛋白原mRNA，细胞的条件培养基中转铁蛋白和白蛋白水平升高。STO饲养层细胞共培养可能有助于其他物种肝细胞的可持续培养。
