Chang S Y, McGary E C, Chang S
Department of Microbial Genetics, Cetus Corporation, Emeryville, California 94608.
J Bacteriol. 1989 Jul;171(7):4071-2. doi: 10.1128/jb.171.7.4071-4072.1989.
We localized the methionine aminopeptidase (map) gene on the Escherichia coli chromosome next to the rpsB gene at min 4. Genetically modified strains with the chromosomal map gene under lac promoter control grew only in the presence of the lac operon inducer isopropyl-beta-thiogalactoside. Thus, methionine aminopeptidase is essential for cell growth.
我们将甲硫氨酸氨基肽酶(map)基因定位在大肠杆菌染色体上,位于4分钟处的rpsB基因旁边。在乳糖启动子控制下带有染色体map基因的转基因菌株仅在乳糖操纵子诱导剂异丙基-β-硫代半乳糖苷存在时才能生长。因此,甲硫氨酸氨基肽酶对细胞生长至关重要。