大肠杆菌K12中氨肽酶N基因的物理图谱
Physical mapping of the gene for aminopeptidase N in Escherichia coli K12.
作者信息
Bally M, Murgier M, Tommassen J, Lazdunski A
出版信息
Mol Gen Genet. 1984;193(1):190-1. doi: 10.1007/BF00327436.
PMID:6140621
Abstract
The pepN gene has been cloned into the multicopy plasmid pBR322. The restriction map of the insert was established and the gene was localized. By comparison with the restriction map of the plasmid pJP30 bearing the ompF region, it has been possible to order the ompF, asnS, and pepN genes. The ompF and asnS genes are contiguous and pepN is separated from asnS by a DNA fragment of about 1.6 kb.
摘要
pepN基因已被克隆到多拷贝质粒pBR322中。确定了插入片段的限制酶切图谱并对该基因进行了定位。通过与携带ompF区域的质粒pJP30的限制酶切图谱进行比较，已能够确定ompF、asnS和pepN基因的顺序。ompF和asnS基因相邻，而pepN与asnS被一个约1.6 kb的DNA片段隔开。
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