Bally M, Murgier M, Lazdunski A
Mol Gen Genet. 1984;195(3):507-10. doi: 10.1007/BF00341454.
The pepN gene, that encodes aminopeptidase N in Escherichia coli, has been cloned into the multicopy plasmid pBR322. Expression of the cloned pepN gene results in overproduction of the enzyme. The restriction map of the 6.7 Kb insert was established and the gene was further localized by analysis of the in vitro constructed delection plasmid and mutant plasmids generated by Tn5 insertions. Chromosome mobilization experiments, using pep-N-lac fusion strains allowed us to infer a clockwise direction of transcription for the pepN gene.
编码大肠杆菌氨肽酶N的pepN基因已被克隆到多拷贝质粒pBR322中。克隆的pepN基因的表达导致该酶的过量产生。建立了6.7 Kb插入片段的限制性图谱，并通过分析体外构建的缺失质粒和由Tn5插入产生的突变体质粒进一步定位了该基因。使用pep-N-lac融合菌株进行的染色体转移实验使我们能够推断出pepN基因的转录方向为顺时针。
