Podtaev Sergey, Stepanov Rodion, Smirnova Elena, Loran Evgenia
Institute of Continuous Media Mechanics, Ural Branch of Russian, Academy of Science, Perm, Russian Federation.
Institute of Continuous Media Mechanics, Ural Branch of Russian, Academy of Science, Perm, Russian Federation; Perm National Research Polytechnic University, Perm, Russian Federation.
Microvasc Res. 2015 Jan;97:109-14. doi: 10.1016/j.mvr.2014.10.003. Epub 2014 Oct 27.
Skin microvessels have proven to be a model to investigate the mechanisms of vascular disease; in particular, endothelial dysfunction. To analyze skin blood flow, high-resolution thermometry can be used because low-amplitude skin temperature oscillations are caused by changes in the tone of skin vessels. The aim of our study was to test the possibilities of wavelet analysis of skin temperature (WAST) for the diagnosis of impaired regulation of microvascular tone in patients with type 2 diabetes. A local heating functional test was used for the assessment of microvascular tone regulation. A control group consisted of healthy male and female volunteers (n=5 each), aged 39.1±5.3years. A group of patients with type 2 diabetes comprised thirteen people, seven men and six women, aged 36 to 51years old (43.2±3.4years). The diagnosis of diabetes was made according to the criteria of the World Health Organization (WHO). The mean disease duration was 7.36±0.88years. Skin temperature oscillations, reflecting intrinsic myogenic activity (0.05-0.14Hz), neurogenic factors (0.02-0.05Hz) and endothelial activity (0.0095-0.02Hz) increase greatly during local heating for healthy subjects. In the group of patients with type 2 diabetes, no statistically significant differences in the amplitudes in the endothelial range were observed. Relative changes in the oscillation amplitudes in patients with type 2 diabetes were markedly lower compared to the control group. The latter indicates that the WAST method enables assessment of the state of vascular tone and the effects of mechanisms responsible for regulation of blood flow in the microvasculature.
皮肤微血管已被证明是研究血管疾病机制的一个模型；特别是内皮功能障碍。为了分析皮肤血流，可以使用高分辨率温度测量法，因为低幅度的皮肤温度振荡是由皮肤血管张力的变化引起的。我们研究的目的是测试皮肤温度小波分析（WAST）在诊断2型糖尿病患者微血管张力调节受损方面的可能性。采用局部加热功能试验来评估微血管张力调节。对照组由健康的男性和女性志愿者组成（各5名），年龄为39.1±5.3岁。一组2型糖尿病患者包括13人，7名男性和6名女性，年龄在36至51岁之间（43.2±3.4岁）。糖尿病的诊断根据世界卫生组织（WHO）的标准进行。平均病程为7.36±0.88年。对于健康受试者，在局部加热期间，反映内在肌源性活动（0.05 - 0.14Hz）、神经源性因素（0.02 - 0.05Hz）和内皮活性（0.0095 - 0.02Hz）的皮肤温度振荡会大幅增加。在2型糖尿病患者组中，在内皮范围内的振幅未观察到统计学上的显著差异。与对照组相比，2型糖尿病患者振荡振幅的相对变化明显更低。后者表明WAST方法能够评估血管张力状态以及负责调节微血管血流的机制的作用。
