使用DiversiLab™基于重复序列的PCR系统对临床和环境来源的大肠杆菌O104分离株进行聚类分析。
Clustering of clinical and environmental Escherichia coli O104 isolates using the DiversiLab™ repetitive sequence-based PCR system.
作者信息
Herbold N M, Clotilde L M, Anderson K M, Kase J, Hartman G L, Himathongkham S, Lin A, Lauzon C R
机构信息
Department of Biological Sciences, California State University, East Bay, 25800 Carlos Bee Blvd., Hayward, CA, 94542, USA.
出版信息
Curr Microbiol. 2015 Mar;70(3):436-40. doi: 10.1007/s00284-014-0728-z. Epub 2014 Dec 2.
The DiversiLab™ rep-PCR system was used to amplify DNA regions of 28 well-characterized Escherichia coli O104 strains to generate a digital DNA fingerprint profile for strain differentiation. E. coli O104 strains from human stools and other sources were examined. The results indicate that this system can cluster similar O104 strains rapidly.
使用DiversiLab™重复聚合酶链反应（rep-PCR）系统扩增28株特征明确的大肠杆菌O104菌株的DNA区域，以生成用于菌株区分的数字DNA指纹图谱。对来自人类粪便和其他来源的大肠杆菌O104菌株进行了检测。结果表明，该系统可快速将相似的O104菌株聚类。
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