使用一种基于重复序列的商业化DiversiLab半自动PCR分型技术来鉴定产CTX-M-15的大肠杆菌克隆ST131。
Using a commercial DiversiLab semiautomated repetitive sequence-based PCR typing technique for identification of Escherichia coli clone ST131 producing CTX-M-15.
作者信息
Pitout Johann D D, Campbell Lorraine, Church Deirdre L, Wang Pauline W, Guttman David S, Gregson Daniel B
机构信息
Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada.
出版信息
J Clin Microbiol. 2009 Apr;47(4):1212-5. doi: 10.1128/JCM.02265-08. Epub 2009 Feb 9.
Abstract
A study was designed to evaluate the ability of the DiversiLab fingerprinting kit, a type of repetitive element PCR (rep-PCR), to identify Escherichia coli clone ST131 producing beta-lactamase CTX-M-15. A set of 53 nonduplicate isolates of extended-spectrum beta-lactamase-producing E. coli underwent rep-PCR, pulsed-field gel electrophoresis, and multilocus sequence typing. The DiversiLab system successfully identified E. coli clone ST131 producing CTX-M-15 and provides a simple standardized typing protocol for monitoring the spread of this clone.
摘要
一项研究旨在评估DiversiLab指纹识别试剂盒（一种重复元件PCR（rep-PCR））鉴定产β-内酰胺酶CTX-M-15的大肠杆菌克隆ST131的能力。对一组53株非重复的产超广谱β-内酰胺酶大肠杆菌进行了rep-PCR、脉冲场凝胶电泳和多位点序列分型。DiversiLab系统成功鉴定了产CTX-M-15的大肠杆菌克隆ST131，并为监测该克隆的传播提供了一种简单的标准化分型方案。
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