Mochizuki M, Konishi S, Ajiki M, Akaboshi T
Nihon Juigaku Zasshi. 1989 Apr;51(2):264-72. doi: 10.1292/jvms1939.51.264.
Four monoclonal antibodies (mAb) against a feline panleukopenia virus (FPLV) TU 1 strain, one of the host range variants of feline parvovirus (FPV), were produced and applied for antigenic analysis of FPLV, canine parvovirus (CPV) and mink enteritis virus (MEV). All mAbs were considered to be directed at epitopes on the virus capsid surface because they neutralized the infectivity and inhibited the hemagglutination (HA) of the homologous virus as well as other FPV strains. They were of the mouse IgG1 type. High antigenic homogeneity among FPLV strains was confirmed by HA-inhibition (HI) test with the mAbs and polyclonal immune sera against FPLV or CPV. But the TU 11 strain of FPLV was antigenically distinguished from the remaining 14 FPLV strains by both the HI test and the micro-neutralization test with one of the mAbs produced. MEV Abashiri strain was found to be antigenically indistinguishable from FPLV. Most of the CPV strains isolated after 1981 were considered to be antigenically different from earlier CPV isolates when some mAbs were applied in the serological tests, confirming the replacement of CPV by an antigenic variant in Japan. However, antigenically different CPVs were detected at the end of 1984 from unrelated epizootics occurred a month apart in the same area.
制备了四种针对猫泛白细胞减少症病毒（FPLV）TU 1株（猫细小病毒（FPV）的宿主范围变异株之一）的单克隆抗体（mAb），并将其用于FPLV、犬细小病毒（CPV）和水貂肠炎病毒（MEV）的抗原分析。所有单克隆抗体均被认为是针对病毒衣壳表面的表位，因为它们能中和同源病毒以及其他FPV毒株的感染性并抑制其血凝作用（HA）。它们属于小鼠IgG1型。通过使用这些单克隆抗体以及针对FPLV或CPV的多克隆免疫血清进行血凝抑制（HI）试验，证实了FPLV毒株之间具有高度的抗原同质性。但是，通过HI试验以及使用所制备的一种单克隆抗体进行的微量中和试验发现，FPLV的TU 11毒株在抗原性上与其余14种FPLV毒株不同。发现MEV阿巴町毒株在抗原性上与FPLV无法区分。当在血清学试验中应用某些单克隆抗体时，1981年以后分离的大多数CPV毒株被认为在抗原性上与早期的CPV分离株不同，这证实了在日本CPV已被一种抗原变异株所取代。然而，在1984年末，在同一地区相隔一个月发生的无关动物流行病中检测到了抗原性不同的CPV。
