Freeman K P, Castro A E, Kautz C E
Vet Microbiol. 1986 Feb;11(1-2):61-8. doi: 10.1016/0378-1135(86)90007-6.
A parvovirus was isolated from the feces of an 8- and 9-month-old steer that died acutely with hemorrhagic diarrhea and microscopic evidence of a coccidial infection. The concurrent intestinal parasitism in this steer appeared to play a role in the development of clinical disease. The viral isolate was identified as a bovine parvovirus (BPV) on the basis of its size (22 nm) and icosahedral morphology, the neutralization of viral cytopathology by antiserum to BPV, a strong immunofluorescent reaction with fluorescein-labeled antiserum to BPV, and the inhibition of viral hemagglutination of guinea-pig erythrocytes by antiserum to BPV. Cell cultures infected with this isolate showed a slight nuclear fluorescent reaction with fluorescein-labeled antiserum to canine parvovirus, suggesting an antigenic relationship to canine parvovirus. Patterns of hemagglutination for this isolate with human erythrocytes from 20 donors of various blood types differed from those obtained with the reference Abinanti strain of BPV. These results indicate that blood from multiple donors of a species may be necessary to confirm the presence or absence of viral hemagglutinating activity with clinical isolates of BPV.
从一头8个月和9个月大的急性死亡的小公牛粪便中分离出一种细小病毒，该小公牛患有出血性腹泻，并有球虫感染的微观证据。这头小公牛同时存在的肠道寄生虫感染似乎在临床疾病的发展中起了作用。根据病毒大小（22纳米）和二十面体形态、抗牛细小病毒血清对病毒细胞病变的中和作用、与荧光素标记的抗牛细小病毒血清的强免疫荧光反应以及抗牛细小病毒血清对豚鼠红细胞病毒血凝的抑制作用，将该病毒分离株鉴定为牛细小病毒（BPV）。用该分离株感染的细胞培养物与荧光素标记的抗犬细小病毒血清显示出轻微的核荧光反应，表明与犬细小病毒存在抗原关系。该分离株对来自20名不同血型供体的人红细胞的血凝模式与用BPV参考阿比纳蒂株获得的模式不同。这些结果表明，可能需要来自一个物种多个供体的血液来确认BPV临床分离株是否存在病毒血凝活性。
