O'Driscoll Daniel, Škrabáková Zuzana, James Kevin J
Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork, Cork, Ireland; PROTEOBIO (Mass Spectrometry Centre), Cork Institute of Technology, Bishopstown, Cork, Ireland.
PROTEOBIO (Mass Spectrometry Centre), Cork Institute of Technology, Bishopstown, Cork, Ireland; Environmental Research Institute, University College Cork, Lee Road, Cork, Ireland.
Toxicon. 2014 Dec 15;92:123-8. doi: 10.1016/j.toxicon.2014.10.012. Epub 2014 Oct 16.
Liquid chromatography multiple tandem mass spectrometry (LC-MS/MS) has been applied to demonstrate that azaspiracid (AZA1), its isomer, AZA6, and its methyl- and demethyl-analogues, AZA2 and AZA3 respectively, are distributed throughout mussel (Mytilus edulis) tissue compartments. Large differences in both the toxin content and the toxin profiles were observed in 20 individual mussels from the same batch. The toxin levels found in the hepatopancreas of mussels were, AZA1 (0.02-5.0 μg/g); AZA2 (0.12-1.9 μg/g), AZA3 (0.06-0.88 μg/g) and AZA6 (0.05-2.0 μg/g). This study also examined the toxin content in mussel tissue compartments and it was found that the gills contributed significantly to the overall level of toxins in mussels. Although polyether toxins are usually concentrated in the hepatopancreas of shellfish this is not always the situation with azaspiracids. The mean distribution of azaspiracids in mussels was; hepatopancreas (60.6%), gills (12.0%) and adductor muscle (27.4%).
液相色谱串联质谱法（LC-MS/MS）已被用于证明azaspiracid（AZA1）、其异构体AZA6以及其甲基化和去甲基化类似物AZA2和AZA3分别分布于贻贝（紫贻贝）的各个组织部分。在同一批次的20个贻贝个体中观察到毒素含量和毒素谱存在很大差异。贻贝肝胰腺中发现的毒素水平为：AZA1（0.02 - 5.0μg/g）；AZA2（0.12 - 1.9μg/g），AZA3（0.06 - 0.88μg/g）和AZA6（0.05 - 2.0μg/g）。本研究还检测了贻贝组织部分中的毒素含量，发现鳃对贻贝中毒素的总体水平有显著贡献。虽然聚醚毒素通常集中在贝类的肝胰腺中，但azaspiracids并非总是如此。azaspiracids在贻贝中的平均分布为：肝胰腺（60.6%）、鳃（12.0%）和闭壳肌（27.4%）。
