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具有单个取代的GBF/Gea突变体在没有BIG/Sec7的情况下仍能维持真菌生长。

GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7.

作者信息

Arst Herbert N, Hernandez-Gonzalez Miguel, Peñalva Miguel A, Pantazopoulou Areti

机构信息

Section of Microbiology, Department of Medicine, Imperial College London, London SW7 2AZ, United Kingdom; Centro de Investigaciones Biológicas, CSIC, Madrid 28040, Spain.

Centro de Investigaciones Biológicas, CSIC, Madrid 28040, Spain.

出版信息

FEBS Lett. 2014 Dec 20;588(24):4799-806. doi: 10.1016/j.febslet.2014.11.014. Epub 2014 Nov 20.

DOI:10.1016/j.febslet.2014.11.014
PMID:25451223
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4266534/
Abstract

Golgi Arf1-guanine nucleotide exchange factors (GEFs) belong to two subfamilies: GBF/Gea and BIG/Sec7. Both are conserved across eukaryotes, but the physiological role of each is not well understood. Aspergillus nidulans has a single member of the early Golgi GBF/Gea-subfamily, geaA, and the late Golgi BIG/Sec7-subfamily, hypB. Both geaA and hypB are essential. hypB5 conditionally blocks secretion. We sought extragenic hypB5 suppressors and obtained geaA1. geaA1 results in Tyr1022Cys within a conserved GBF/Gea-specific S(Y/W/F)(L/I) motif in GeaA. This mutation alters GeaA localization. Remarkably, geaA1 suppresses hypBΔ, indicating that a single mutant Golgi Arf1-GEF suffices for growth.

摘要

高尔基体Arf1鸟嘌呤核苷酸交换因子（GEF）属于两个亚家族：GBF/Gea和BIG/Sec7。两者在真核生物中都保守，但各自的生理作用尚未完全了解。构巢曲霉有早期高尔基体GBF/Gea亚家族的单个成员geaA，以及晚期高尔基体BIG/Sec7亚家族的hypB。geaA和hypB都是必需的。hypB5条件性地阻断分泌。我们寻找hypB5的基因外抑制子并获得了geaA1。geaA1导致GeaA中保守的GBF/Gea特异性S(Y/W/F)(L/I)基序内的Tyr1022Cys。该突变改变了GeaA的定位。值得注意的是，geaA1抑制hypBΔ，表明单个突变的高尔基体Arf1-GEF足以支持生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/b7007a31aa3f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/b27c323b7aab/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/699ee5048ecb/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/07026e8c76b9/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/8b1888d4d859/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/b7007a31aa3f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/b27c323b7aab/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/699ee5048ecb/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/07026e8c76b9/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/8b1888d4d859/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b00/4266534/b7007a31aa3f/fx1.jpg

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