个性化饮食和益生菌补充剂对炎症、营养参数及肠道微生物群的影响——“RISTOMED项目”：健康老年人的随机对照试验

Impact of personalized diet and probiotic supplementation on inflammation, nutritional parameters and intestinal microbiota - The "RISTOMED project": Randomized controlled trial in healthy older people.

作者信息

Valentini Luzia, Pinto Alessandro, Bourdel-Marchasson Isabelle, Ostan Rita, Brigidi Patrizia, Turroni Silvia, Hrelia Silvana, Hrelia Patrizia, Bereswill Stefan, Fischer André, Leoncini Emanuela, Malaguti Marco, Blanc-Bisson Christéle, Durrieu Jessica, Spazzafumo Liana, Buccolini Fabio, Pryen Florence, Donini Lorenzo Maria, Franceschi Claudio, Lochs Herbert

机构信息

Dept Gastroenterology and Hepatology, Charité-Universitätsmedizin Berlin, CCM, Charitéplatz 1, 10117 Berlin, Germany.

Experimental Medicine Department, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.

出版信息

Clin Nutr. 2015 Aug;34(4):593-602. doi: 10.1016/j.clnu.2014.09.023. Epub 2014 Oct 8.

DOI:10.1016/j.clnu.2014.09.023

PMID:25453395

Abstract

OBJECTIVES

To assess the impact of a personalized diet, with or without addition of VSL#3 preparation, on biomarkers of inflammation, nutrition, oxidative stress and intestinal microbiota in 62 healthy persons aged 65-85 years.

DESIGN

Open label, randomized, multicenter study.

PRIMARY ENDPOINT

High-sensitivity C-reactive protein.

SETTING

Community.

INTERVENTIONS

Eight week web-based dietary advice (RISTOMED platform) alone or with supplementation of VSL#3 (2 capsules per day). The RISTOMED diet was optimized to reduce inflammation and oxidative stress.

MEASUREMENTS

Blood and stool samples were collected on days 1 and 56.

RESULTS

Diet alone reduced ESR (p = 0.02), plasma levels of cholesterol (p < 0.01) and glucose (p = 0.03). Addition of VSL#3 reduced ESR (p = 0.05) and improved folate (p = 0.007), vitamin B12 (p = 0.001) and homocysteine (p < 0.001) plasma levels. Neither intervention demonstrated any further effects on inflammation. Subgroup analysis showed 40 participants without signs of low-grade inflammation (hsCRP<3 mg/l, subgroup 1) and 21 participants with low-grade inflammation at baseline (hsCRP≥3 mg/l, subgroup 2). In subgroup 2 addition of VSL#3 increased bifidobacteria (p = 0.005) in more participants and improved both folate (p = 0.015) and vitamin B12 (p = 0.035) levels compared with subgroup 1. The increases were positively correlated to the change in the bifidobacteria concentration for folate (p = 0.023) and vitamin B12 (p = 0.001). As expected change in homocysteine correlated negatively to change in folate (r = -0.629, p = 0.002) and vitamin B12 (r = -0.482, p = 0.026).

CONCLUSIONS

Addition of VSL#3 increased bifidobacteria and supported adequate folate and vitamin B12 concentrations in subjects with low-grade inflammation. Decrease in homocysteine with VSL#3 was clinically relevant. suggesting protective potentials for aging-associated conditions, e.g. cardiovascular or neurological diseases. ClinicalTrials.gov: NCT01069445-NCT01179789.

摘要

目的

评估个性化饮食（添加或不添加VSL#3制剂）对62名65 - 85岁健康人群炎症、营养、氧化应激生物标志物及肠道微生物群的影响。

设计

开放标签、随机、多中心研究。

主要终点

高敏C反应蛋白。

地点

社区。

干预措施

单独进行为期八周的基于网络的饮食建议（RISTOMED平台）或补充VSL#3（每日2粒胶囊）。RISTOMED饮食经过优化以减轻炎症和氧化应激。

测量指标

在第1天和第56天采集血液和粪便样本。

结果

单纯饮食降低了红细胞沉降率（p = 0.02）、血浆胆固醇水平（p < 0.01）和血糖水平（p = 0.03）。添加VSL#3降低了红细胞沉降率（p = 0.05），并改善了叶酸（p = 0.007）、维生素B12（p = 0.001）和同型半胱氨酸的血浆水平（p < 0.001）。两种干预措施均未显示对炎症有任何进一步影响。亚组分析显示，40名参与者无低度炎症迹象（高敏C反应蛋白<3mg/l，亚组1），21名参与者在基线时有低度炎症（高敏C反应蛋白≥3mg/l，亚组2）。在亚组2中，与亚组1相比，添加VSL#3使更多参与者的双歧杆菌增加（p = 0.005），并改善了叶酸（p = 0.015）和维生素B12水平（p = 0.035）。这些增加与叶酸（p = 0.023）和维生素B12（p = 0.001）的双歧杆菌浓度变化呈正相关。正如预期的那样，同型半胱氨酸的变化与叶酸变化呈负相关（r = -0.629，p = 0.002）和维生素B12变化呈负相关（r = -0.482，p = 0.026）。