Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra, 416 004, India.
Ayurvedic practitioner, Mangeshkar Nagar, Kolhapur, India.
In Vitro Cell Dev Biol Anim. 2022 May;58(5):376-383. doi: 10.1007/s11626-022-00690-x. Epub 2022 Jun 6.
Type 1 diabetes mellitus is an autoimmune disorder leading to loss of beta cells. There is a dire need to inhibit apoptosis and induce regeneration of new beta cells. There are plants in the Indian medicine system having the potential for rejuvenation. In the present study, we have attempted to evaluate the capacity of aqueous extract of Tinospora cordifolia to regenerate beta cells from PANC-1 ductal cells. After differentiation, the characterization of β-cell phenotype was carried out using dithizone and Gomori's staining and further confirmed by mRNA expression study of insulin, Pdx-1, and carbonic anhydrase-9. Insulin production was estimated with ELISA. Aqueous extract of Tinospora cordifolia at 15 μg/ml concentration can effectively induce differentiation of PANC-1 cells into beta cells. The morphological observations showed brownish-colored dithizone and purple-colored Gomori's staining. The β-cells demonstrated significant mRNA expression of insulin and Pdx-1 and downregulation of carbonic anhydrase-9. The functionality of beta cells was demonstrated by 1.5-fold increase in insulin secretion in response to high glucose. Tinospora cordifolia has potential to differentiate PANC-1 ductal cells into functional beta cells and can be a lead towards non-invasive treatment of type 1 diabetes mellitus.
1 型糖尿病是一种自身免疫性疾病，导致β细胞丧失。目前迫切需要抑制细胞凋亡并诱导新的β细胞再生。印度医学体系中有一些植物具有恢复活力的潜力。在本研究中，我们试图评估三叶鬼针草水提物从 PANC-1 导管细胞再生β细胞的能力。分化后，使用二硫腙和 Gomori 染色对β细胞表型进行特征描述，并通过胰岛素、Pdx-1 和碳酸酐酶-9 的 mRNA 表达研究进一步证实。用 ELISA 测定胰岛素的产生。在 15μg/ml 浓度下，三叶鬼针草水提取物可有效诱导 PANC-1 细胞分化为β细胞。形态学观察显示出棕色的二硫腙和紫色的 Gomori 染色。β细胞表现出胰岛素和 Pdx-1 的显著 mRNA 表达，并下调碳酸酐酶-9。β细胞的功能通过对高葡萄糖的胰岛素分泌增加 1.5 倍来证明。三叶鬼针草具有将 PANC-1 导管细胞分化为功能性β细胞的潜力，可成为 1 型糖尿病非侵入性治疗的潜在方法。
