Holmberg C, Rutberg B
Department of Microbiology, University of Lund, Sweden.
FEMS Microbiol Lett. 1989 Apr;49(2-3):151-5. doi: 10.1016/0378-1097(89)90030-x.
A 3.5 kb fragment of Bacillus subtilis DNA which contains wild type alleles of mutations in glpK (glycerol kinase) and glpD (glycerol-3-phosphate [G3P] dehydrogenase) was cloned in plasmid pHV32 in Escherichia coli. The cloned fragment expresses glycerol kinase in B. subtilis mutants carrying the mutations glpK11 and recE4 after induction with glycerol or G3P whereas it does not express G3P dehydrogenase. The cloned fragment thus contains the complete glpK but probably only part of glpD.
一段3.5 kb的枯草芽孢杆菌DNA片段被克隆到大肠杆菌的质粒pHV32中，该片段包含glpK（甘油激酶）和glpD（甘油-3-磷酸[G3P]脱氢酶）突变的野生型等位基因。在用甘油或G3P诱导后，该克隆片段在携带glpK11和recE4突变的枯草芽孢杆菌突变体中表达甘油激酶，而不表达G3P脱氢酶。因此，该克隆片段包含完整的glpK，但可能只包含glpD的一部分。
