Langlois B E, Harmon R J, Akers K, Aaron D K
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215.
J Clin Microbiol. 1989 May;27(5):1127-9. doi: 10.1128/jcm.27.5.1127-1129.1989.
A greater percentage of DNase-positive strains was detected with DNase test agar than with DNase test agar containing 0.005% methyl green or 0.005% toluidine blue (P less than 0.01). No significant differences were obtained in the percentage of phosphatase-positive strains with the four methods compared. On the basis of ease of use, P agar containing para-nitrophenylphosphate disodium (0.495 mg/ml) would be the preferred method for determining phosphatase activity of staphylococci.
与含有0.005%甲基绿或0.005%甲苯胺蓝的DNase试验琼脂相比，用DNase试验琼脂检测到的DNase阳性菌株百分比更高（P<0.01）。比较这四种方法，磷酸酶阳性菌株的百分比没有显著差异。基于使用的便利性，含有对硝基苯磷酸二钠（0.495mg/ml）的P琼脂将是测定葡萄球菌磷酸酶活性的首选方法。
