Johansson E, Welinder-Olsson C, Gilljam M, Pourcel C, Lindblad A
Clinical Microbiology, Sahlgrenska University Hospital, P.O.B. 7193, SE 402 34 Gothenburg, Sweden; Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, SE 405 30 Gothenburg, Sweden.
Gothenburg CF Centre, Sweden; Respiratory Medicine, Bruna Stråket 11b, Sahlgrenska University Hospital, SE 413 45 Gothenburg, Sweden; Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, SE 405 30 Gothenburg, Sweden.
J Cyst Fibros. 2015 May;14(3):353-60. doi: 10.1016/j.jcf.2014.09.016. Epub 2014 Oct 30.
Genotyping of Pseudomonas aeruginosa (P.a) is used for surveillance at our CF clinic.
P.a from 1999 to 2012 were analysed, using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable number of tandem repeats analysis (MLVA).
Among 232 isolates from 104 patients, we identified 78 unique strains, of which 56 were isolated from individual patients. The B-clone was isolated from 13 patients and the camp transmission clone J-strains from 8 patients at the start of the study. There was no indication of transmission within the clinic. PFGE and MLVA clone identification was in 91% agreement. For patients who provided more than 2 P.a isolates, similar strains were identified over time for 45/49 chronically- and for 6/16 intermittently-colonized patients despite, periods of no detectable P.a after eradication therapy.
Analyses revealed high genotypic diversity, acceptable outcome of eradication therapy and no indication of cross-infection at the CF centre.
铜绿假单胞菌（P.a）基因分型用于我们囊性纤维化（CF）诊所的监测。
对1999年至2012年的铜绿假单胞菌进行分析，采用脉冲场凝胶电泳（PFGE）和多位点可变数目串联重复序列分析（MLVA）。
在104例患者的232株分离株中，我们鉴定出78株独特菌株，其中56株从个体患者中分离得到。在研究开始时，B克隆从13例患者中分离得到，营地传播克隆J菌株从8例患者中分离得到。没有迹象表明诊所有传播情况。PFGE和MLVA克隆鉴定的一致性为91%。对于提供超过2株铜绿假单胞菌分离株的患者，尽管在根除治疗后有一段时间检测不到铜绿假单胞菌，但在45/49例慢性定植和6/16例间歇性定植患者中，随着时间的推移鉴定出了相似菌株。
分析显示基因型多样性高、根除治疗效果可接受且CF中心无交叉感染迹象。
