Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, London, UK.
Clin Microbiol Infect. 2010 Aug;16(8):1111-6. doi: 10.1111/j.1469-0691.2009.03049.x. Epub 2009 Sep 3.
Repeat numbers at nine variable-number tandem-repeat (VNTR) loci were determined for 177 isolates of Pseudomonas aeruginosa representing 77 strains distinguished by pulsed-field gel electrophoresis (PFGE). Eight loci provided for discrimination similar to that provided by PFGE, with variation at the ninth locus (ms61) sometimes allowing discrimination within a PFGE-defined type. The Liverpool and Midlands 1 strains, which are common among patients with cystic fibrosis in the UK, could be unambiguously identified by their characteristic VNTR profiles. In rare cases, the repeat number at the ninth locus alone provided discrimination among isolates that were distinct according to PFGE. In each case, the two isolates shared the same bla(OXA-50-like) allele and belonged to the same oprD sequence type group, supporting the VNTR results in suggesting that they are similar.
对 177 株代表 77 种不同菌株的铜绿假单胞菌分离株进行了 9 个可变数串联重复（VNTR）位点的重复数测定，这些菌株通过脉冲场凝胶电泳（PFGE）进行了区分。8 个位点提供的分辨率与 PFGE 提供的分辨率相似，第 9 个位点（ms61）的变异有时允许在 PFGE 定义的类型内进行区分。利物浦和米德兰 1 株是英国囊性纤维化患者中常见的菌株，可以通过其特征性的 VNTR 图谱明确识别。在极少数情况下，仅第九个位点的重复数就可以在根据 PFGE 区分的分离株之间进行区分。在每种情况下，两个分离株都具有相同的 bla（OXA-50 样）等位基因，并且属于相同的 oprD 序列类型组，这支持了 VNTR 结果，表明它们是相似的。
