Murthy S C, Trimble J J, Desrosiers R C
New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772.
J Virol. 1989 Aug;63(8):3307-14. doi: 10.1128/JVI.63.8.3307-3314.1989.
Analysis of a 5,549-base-pair sequence at the left end of herpesvirus saimiri unique (L-) DNA revealed two open reading frames and genes for five small nuclear U RNAs (herpesvirus saimiri U RNAs). Replication-competent deletion mutants were constructed in order to assess the importance of these genetic features for transformation by this oncogenic herpesvirus. Although not required for replication, one of the open reading frames was found to be required for immortalization of marmoset T lymphocytes into continuously growing cell lines. The protein predicted by this reading frame (STP; saimiri transformation-associated protein) has a highly hydrophobic stretch of 26 amino acids sufficient for a membrane-spanning domain near its carboxy terminus; this domain is immediately preceded by a sequence appropriate for formation of a metal-binding domain (His X2 His X6 Cys X2 Cys, where Xs are other amino acids). One of two poly(A)+ RNAs that could encode STP is bicistronic, while the other has a long 5' untranslated region (approximately 1.5 kilobases). Although some analogies can be drawn between STP and LMP (lymphocyte membrane protein) of Epstein-Barr virus, STP is not related in sequence to LMP.
对猴疱疹病毒独特（L-）DNA左端一段5549个碱基对的序列分析揭示了两个开放阅读框以及五个小核U RNA（猴疱疹病毒U RNA）的基因。构建了具有复制能力的缺失突变体，以评估这些遗传特征对于这种致癌疱疹病毒转化的重要性。尽管复制不需要其中一个开放阅读框，但发现它是将狨猴T淋巴细胞永生化成为持续生长的细胞系所必需的。由这个阅读框预测的蛋白质（STP；猴病毒转化相关蛋白）在其羧基末端附近有一段由26个氨基酸组成的高度疏水序列，足以形成一个跨膜结构域；该结构域之前紧接着一段适合形成金属结合结构域的序列（His X2 His X6 Cys X2 Cys，其中X为其他氨基酸）。两个可编码STP的聚腺苷酸化（poly(A)+）RNA之一是双顺反子的，而另一个有一个长的5'非翻译区（约1.5千碱基）。尽管STP与爱泼斯坦-巴尔病毒的LMP（淋巴细胞膜蛋白）之间可以进行一些类比，但STP与LMP在序列上没有关系。
