Shimada K
Nihon Hifuka Gakkai Zasshi. 1989 Feb;99(2):101-10.
Immunohistochemically, the presence of lysozyme (LZ) has been detected by the antibody against human LZ in cytoplasm of cells from granulomatous and histiocyte-proliferative skin diseases. To detect LZ in these cells morphologically, I have done electron microscopic observations of the following skin diseases; sarcoidosis, lupus vulgaris, lupus miliaris disseminatus faciei (LMDF), tattoo granuloma, lichen nitidus, foreign body granuloma, granuloma annulare, xanthelasma, xanthoma tuberosum, xanthoma planum, juvenile xanthogranuloma, giant cell tumor of tendon sheath, dermatofibroma, malignant fibrous histiocytoma, dermatofibrosarcoma protuberans, granulation tissue of burn, hypertrophic scar, and histiocytosis X. From both the immunohistochemical and the electron microscopic features it was concluded that a) immunohistochemically LZ-positive cells from lesions of sarcoidosis, lupus vulgaris, LMDF and tattoo granuloma had a number of electron-lucent bodies (ELB) or microvesicles in their cytoplasm, b) lichen nitidus and xanthoma tuberosum had few LZ-positive cells and the ELB were not observed, and c) the other diseases were LZ-negative, and the ELB were also absent. It is suggested that LZ is present in the ELB which are observed electron microscopically.
免疫组织化学方法中，通过抗人溶菌酶（LZ）抗体已在肉芽肿性和组织细胞增生性皮肤病的细胞胞质中检测到溶菌酶（LZ）的存在。为了从形态学上检测这些细胞中的LZ，我对以下皮肤病进行了电子显微镜观察：结节病、寻常狼疮、面部播散性粟粒性狼疮（LMDF）、纹身肉芽肿、光泽苔藓、异物肉芽肿、环状肉芽肿、睑黄瘤、结节性黄瘤、扁平黄瘤、幼年性黄色肉芽肿、腱鞘巨细胞瘤、皮肤纤维瘤、恶性纤维组织细胞瘤、隆突性皮肤纤维肉瘤、烧伤肉芽组织、肥厚性瘢痕和组织细胞增生症X。从免疫组织化学和电子显微镜特征得出以下结论：a）结节病、寻常狼疮、LMDF和纹身肉芽肿病变中的免疫组织化学LZ阳性细胞在其胞质中有许多电子透明体（ELB）或微泡；b）光泽苔藓和结节性黄瘤中LZ阳性细胞很少，未观察到ELB；c）其他疾病为LZ阴性，也未观察到ELB。提示LZ存在于电子显微镜下观察到的ELB中。
