Bezgin Tugba, Yilmaz Ayca Dilara, Celik Burcu Nihan, Kolsuz Mehmet Eray, Sonmez Hayriye
Department of Pedodontics, Faculty of Dentistry, Ankara University, Ankara, Turkey.
Molecular Biology Laboratory, Faculty of Dentistry, Ankara University, Ankara, Turkey.
J Endod. 2015 Jan;41(1):36-44. doi: 10.1016/j.joen.2014.10.004. Epub 2014 Nov 12.
Current research is concerned with discovering better scaffolds for use in regenerative endodontic treatment. This study aimed to clinically and radiographically evaluate the efficacy of platelet-rich plasma (PRP) used as a scaffold in regenerative endodontic treatment and compare it with that of a conventional blood clot (BC) scaffold.
A total of 20 necrotic, single-rooted immature teeth were randomly distributed into 2 groups. After disinfecting the root canal space with triple antibiotic paste (1:1:1 ciprofloxacin, metronidazole, and cefaclor), a tissue scaffold was created by using either PRP or BC and covered with white mineral trioxide aggregate. Clinical and radiographic follow-up examinations were performed once every 3 months during an 18-month period. Differences in root area were calculated from preoperative and postoperative radiographs. Fisher exact and Mann-Whitney U tests were used to evaluate differences between groups, with P value <.05 considered to be statistically significant.
All 20 teeth were clinically asymptomatic during 18-month follow-up period; however, 1 tooth in the BC group exhibited periapical pathosis and was judged radiographically unsuccessful. Complete apical closure was observed in a mean of 8.1 months in the PRP group compared with 9 months in the BC group. The PRP group exhibited 9.86% increase in root area, compared with 12.6% increase in the BC group. The difference in success rates between the groups was not statistically significant (P > .05).
PRP successfully created a scaffold for regenerative endodontic treatment; however, treatment outcomes did not differ significantly between PRP and conventional BC scaffold.
当前的研究致力于发现用于再生性牙髓治疗的更好支架。本研究旨在通过临床和影像学评估富血小板血浆（PRP）作为再生性牙髓治疗支架的疗效，并将其与传统血凝块（BC）支架进行比较。
将20颗坏死的单根未成熟恒牙随机分为2组。用三联抗生素糊剂（环丙沙星、甲硝唑和头孢克洛按1:1:1比例混合）对根管进行消毒后，分别使用PRP或BC创建组织支架，并用白色矿物三氧化物聚合体覆盖。在18个月的时间里，每3个月进行一次临床和影像学随访检查。根据术前和术后的X线片计算牙根面积的差异。采用Fisher精确检验和Mann-Whitney U检验评估组间差异，P值<.05被认为具有统计学意义。
在18个月的随访期内，所有20颗牙齿临床均无症状；然而，BC组有1颗牙齿出现根尖周病变，影像学判定治疗失败。PRP组平均在8.1个月时观察到根尖完全闭合，而BC组为9个月。PRP组牙根面积增加了9.86%，BC组增加了12.6%。两组成功率的差异无统计学意义（P>.05）。
PRP成功为再生性牙髓治疗创建了支架；然而，PRP与传统BC支架的治疗效果无显著差异。
