Yang Wenxiang, Liu Ji, Zheng Yanhua, Qu Jingjing, Tang Xiaoqiao, Bai Hong, Liu Chunxia, Fan Bolin
Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China.
Center for Food Evaluation, State Administration for Market Regulation, Beijing 10070, China.
Heliyon. 2022 Oct 4;8(10):e10907. doi: 10.1016/j.heliyon.2022.e10907. eCollection 2022 Oct.
The purpose of this study was to evaluate the safety of rhubarb extract.
SD rats were treated with rhubarb extract at 0, 101, 405 and 1620 mg/kg/day for 52 weeks. food consumption and body weights were recorded. Blood and urine samples were collected for serum biochemical evaluation and urinalysis, and organ tissues were collected for histopathological examination.
The rats of 1620 mg/kg group developed diarrhea symptoms with dark brown loose stool after exposure; decreased body weight and increased food consumption were observed in the 1620 mg/kg and 405 mg/kg groups; urine WBC and NIT was significantly increased in the male and female rats of 1620 mg/kg group, and the urine pH was decreased in male rats of 1620 mg/kg group; renal tubular pigmentation was observed in the 1620 mg/kg group.
The NOAEL of rhubarb extract on chronic toxicity (52 weeks) of Sprague-Dawley rats was 101 mg/kg in female and 94 mg/kg in male, and the LOAEL was 408 mg/kg in female and 381 mg/kg in male. The target organ of toxicity was the kidney, and the target cells was tubular epithelial cells.
本研究旨在评估大黄提取物的安全性。
将SD大鼠分别用0、101、405和1620mg/kg/天的大黄提取物处理52周。记录食物消耗量和体重。采集血液和尿液样本进行血清生化评估和尿液分析，并采集器官组织进行组织病理学检查。
1620mg/kg组大鼠在暴露后出现腹泻症状，粪便呈深褐色稀便；1620mg/kg组和405mg/kg组观察到体重下降和食物消耗量增加；1620mg/kg组雄性和雌性大鼠尿液白细胞和亚硝酸盐显著增加，1620mg/kg组雄性大鼠尿液pH值降低；1620mg/kg组观察到肾小管色素沉着。
大黄提取物对Sprague-Dawley大鼠慢性毒性（52周）的无观察到有害作用水平（NOAEL）雌性为101mg/kg，雄性为94mg/kg，最低观察到有害作用水平（LOAEL）雌性为408mg/kg，雄性为381mg/kg。毒性的靶器官是肾脏，靶细胞是肾小管上皮细胞。
