Rudys Romualdas, Denkovskij Jaroslav, Kirdaitė Gailutė, Bagdonas Saulius
Biophotonics Group of Laser Research Center, Faculty of Physics, Vilnius University, Sauletekio 9, bldg. 3, Vilnius LT-10222, Lithuania; State Research Institute Centre for Innovative Medicine, Zygimantu 9, Vilnius LT-01102, Lithuania.
State Research Institute Centre for Innovative Medicine, Zygimantu 9, Vilnius LT-01102, Lithuania.
J Photochem Photobiol B. 2014 Dec;141:228-34. doi: 10.1016/j.jphotobiol.2014.10.003. Epub 2014 Oct 17.
To compare the accumulation of protoporphyrin IX between synoviocytes of patients with rheumatoid arthritis (RA) or osteoarthritis (OA) and cartilage explants (CE) as well as chondrons of patients with OA after the application of 5-aminolevulinic acid (ALA) or its methyl ester (ALA-Me).
Samples of synovial and cartilage tissues were obtained from joint replacement surgeries. The accumulation of PpIX was determined by measuring fluorescence spectra from 2 × 10(5) synoviocytes or chondrons suspended in a glass tube or directly from CE surface after 2, 4, 8 and 24h of incubation with ALA or ALA-Me.
No differences were found between the average fluorescence intensity values of PpIX in synoviocytes of patients with RA and OA. These values were non-significantly higher after incubation with ALA in comparison with ALA-Me at almost all time points. The average fluorescence intensity of PpIX in CE and chondrons was about ten times lower than in synoviocytes. The presence of preparation of hyaluronic acid (HA) significantly enhanced PpIX induction in chondrons versus treatment only with ALA.
A potential for the selective synovial sensitization with endogenous PpIX in comparison with cartilage tissue has been demonstrated in vitro after application of ALA or ALA-Me.
比较类风湿关节炎（RA）或骨关节炎（OA）患者的滑膜细胞与软骨外植体（CE）以及OA患者的软骨细胞在应用5-氨基乙酰丙酸（ALA）或其甲酯（ALA-Me）后原卟啉IX的蓄积情况。
滑膜和软骨组织样本取自关节置换手术。在与ALA或ALA-Me孵育2、4、8和24小时后，通过测量悬浮于玻璃管中的2×10⁵个滑膜细胞或软骨细胞的荧光光谱，或直接测量CE表面的荧光光谱，来确定PpIX的蓄积情况。
RA和OA患者滑膜细胞中PpIX的平均荧光强度值之间未发现差异。在几乎所有时间点，与ALA-Me相比，用ALA孵育后这些值无显著升高。CE和软骨细胞中PpIX的平均荧光强度比滑膜细胞低约十倍。与仅用ALA处理相比，透明质酸（HA）制剂的存在显著增强了软骨细胞中PpIX的诱导。
应用ALA或ALA-Me后，体外实验已证明与软骨组织相比，内源性PpIX对滑膜具有选择性致敏的潜力。
