Goodlad R A, Ratcliffe B, Fordham J P, Wright N A
Department of Histopathology, Royal Postgraduate Medical School, London.
Gut. 1989 Jun;30(6):820-5. doi: 10.1136/gut.30.6.820.
The aim of the present experiment was to investigate the role of hind gut fermentation in the proliferative response of the intestinal epithelium to dietary fibre. We have previously shown that refeeding starved rats with an elemental diet supplemented with fermentable dietary fibre (but not inert bulk) is capable of stimulating intestinal epithelial cell proliferation throughout the gastrointestinal tract. Three groups of 10 germ free (GF) rats and three groups of 10 conventional (CV) rats, were used. All groups were starved for three days and then refed for two days with either an elemental diet (Flexical); Flexical plus 30% kaolin; or Flexical plus 30% of a fibre mixture. Cell production was determined by the accumulation of vincristine arrested metaphases in microdissected crypts. There was no significant difference between refeeding the rats with an elemental diet alone or with kaolin supplementation, however, the addition of fibre in CV rats was associated with a significant increase in intestinal crypt cell production rate in both the small intestine (p less than 0.01) and the colon (p less than 0.001). This marked proliferative effects of fibre was abolished in the GF rats. It can be concluded that it is the products of hind gut fermentation, not fibre per se that stimulate intestinal epithelial cell proliferation in the colon and small intestine.
本实验的目的是研究后肠发酵在肠道上皮对膳食纤维增殖反应中的作用。我们之前已经表明，用补充了可发酵膳食纤维（而非惰性填充物）的要素膳重新喂养饥饿的大鼠，能够刺激整个胃肠道的肠上皮细胞增殖。使用了三组10只无菌（GF）大鼠和三组10只普通（CV）大鼠。所有组均饥饿三天，然后用要素膳（Flexical）、Flexical加30%高岭土或Flexical加30%纤维混合物重新喂养两天。通过在显微解剖的隐窝中积累长春新碱阻滞的中期来测定细胞生成。单独用要素膳或添加高岭土重新喂养大鼠之间没有显著差异，然而，在CV大鼠中添加纤维与小肠（p<0.01）和结肠（p<0.001）的肠隐窝细胞生成率显著增加有关。纤维的这种显著增殖作用在GF大鼠中被消除。可以得出结论，刺激结肠和小肠肠上皮细胞增殖的是后肠发酵产物，而非纤维本身。
