Guo Xiao-Yan, Dong Lei, Qin Bin, Jiang Jiong, Shi A-Meng
Xiao-Yan Guo, Lei Dong, Bin Qin, Jiong Jiang, A-Meng Shi, Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710003, Shaanxi Province, China.
World J Gastroenterol. 2014 Nov 28;20(44):16702-6. doi: 10.3748/wjg.v20.i44.16702.
To investigate the expression of gastrokine 1 (GKN1) in normal gastric mucosa, precancerous lesions and gastric cancer tissues, and to analyse its correlations with tumour site and pathological pattern.
Thirty gastric cancer patients (12 cases of diffuse type and 18 cases of intestinal type), 13 atrophic gastritis patients and 15 healthy volunteers with almost normal gastric mucosa (superficial gastritis) were enrolled in this study. Helicobacter pylori (H. pylori) infection was examined in all subjects. All gastric mucosa biopsy specimens were obtained. Cancer-adjacent specimens were taken from corresponding gastric cancer patients. Immunohistochemistry and real-time PCR were performed to determine the expressions of the GKN1 protein and mRNA, respectively.
H. pylori infection had no significant association with age, gender, tumour site or pathological pattern in all subjects. Compared with the superficial gastritis and atrophic gastritis groups, the expression of GKN1 protein (P = 0.011) and mRNA (P < 0.001) in gastric cancer was significantly decreased. The GKN1 mRNA level in diffuse type gastric cancer was significantly lower than in intestinal type gastric cancer (0.296 ± 0.076 vs 0.525 ± 0.164, P < 0.001).
Compared with almost normal gastric mucosa, GKN1 expression in the gastric mucosa of gastric cancer patients is decreased; this is associated with progression and prognosis of gastric cancer.
研究胃动素1（GKN1）在正常胃黏膜、癌前病变及胃癌组织中的表达情况，并分析其与肿瘤部位及病理类型的相关性。
本研究纳入30例胃癌患者（弥漫型12例，肠型18例）、13例萎缩性胃炎患者及15例胃黏膜基本正常（浅表性胃炎）的健康志愿者。检测所有受试者的幽门螺杆菌（H. pylori）感染情况。获取所有胃黏膜活检标本。从相应胃癌患者中获取癌旁标本。分别采用免疫组织化学和实时荧光定量PCR检测GKN1蛋白和mRNA的表达。
在所有受试者中，H. pylori感染与年龄、性别、肿瘤部位或病理类型均无显著相关性。与浅表性胃炎和萎缩性胃炎组相比，胃癌组织中GKN1蛋白表达（P = 0.011）及mRNA表达（P < 0.001）均显著降低。弥漫型胃癌的GKN1 mRNA水平显著低于肠型胃癌（0.296 ± 0.076 vs 0.525 ± 0.164，P < 0.001）。
与胃黏膜基本正常者相比，胃癌患者胃黏膜中GKN1表达降低；这与胃癌的进展及预后相关。
