Mirzaei Khadijeh, Hossein-nezhad Arash, Keshavarz Seyed Ali, Koohdani Fariba, Eshraghian Mohammad Reza, Saboor-Yaraghi Ali Akbar, Hosseini Saeed, Chamari Maryam, Zareei Mahnaz, Djalali Mahmoud
Cellular and Molecular Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
Tehran University of Medical Sciences, Tehran, Iran; Department of Medicine, Section of Endocrinology, Nutrition, and Diabetes, Vitamin D, Skin and Bone Research Laboratory, Boston University Medical Center, Boston, MA, United States.
Diabetes Metab Syndr. 2015 Oct-Dec;9(4):292-8. doi: 10.1016/j.dsx.2014.04.010. Epub 2014 May 25.
Nesfatin-1 identified neuroendocrine peptide is involved in regulation of homeostasis via modulation of metabolism, energy homeostasis and food intake. We aimed to investigate the associations of circulating nesfatin-1 level with food intake, body composition and resting metabolic rate (RMR) and also examine the correlation between circulating peroxisome proliferator-activated receptor gamma (PPARγ) and nesfatin-1 levels in obese and morbid obese subjects.
A total of 96 obese subjects (including 18 morbid obese subjects) were participated in the current cross-sectional study. We assessed the body composition with the use of Body Composition Analyzer. RMR was measured by means of the MetaCheck™, an instrument designed to measure RMR using indirect calorimetry. All baseline blood samples were obtained following an overnight fasting. Plasma concentrations of nesfatin-1 and circulating PPARγ were measured with the use of an ELISA method. Statistical analyses were performed using SPSS.
We found significant associations between fat percent and circulating nesfatin-1 in obese and morbid obese subjects. There was main association between circulating nesfatin-1 and PPARγ concentration in obese subjects and it was more strong association in morbid obese participants. There was marginally significant differences between percent predicted RMR between different categorized nesfatin-1 levels. There were also higher intakes of calorie, carbohydrate and protein in obese group who had lower concentration of nesfatin-1.
Our data indicated the fat percent as main determinant factor in circulating nesfatin-1 level. It appears nesfatin-1 and PPARγ might be concurrently involved in adipogenesis pathway.
已确定神经内分泌肽Nesfatin-1通过调节新陈代谢、能量平衡和食物摄入量参与体内稳态的调节。我们旨在研究循环Nesfatin-1水平与食物摄入量、身体成分和静息代谢率（RMR）之间的关联，并检测肥胖和病态肥胖受试者循环中过氧化物酶体增殖物激活受体γ（PPARγ）与Nesfatin-1水平之间的相关性。
共有96名肥胖受试者（包括18名病态肥胖受试者）参与了本次横断面研究。我们使用身体成分分析仪评估身体成分。通过MetaCheck™测量RMR，这是一种设计用于通过间接量热法测量RMR的仪器。所有基线血样均在空腹过夜后采集。使用酶联免疫吸附测定（ELISA）方法测量血浆Nesfatin-1浓度和循环PPARγ。使用SPSS进行统计分析。
我们发现肥胖和病态肥胖受试者的脂肪百分比与循环Nesfatin-1之间存在显著关联。肥胖受试者循环Nesfatin-1与PPARγ浓度之间存在主要关联，在病态肥胖参与者中这种关联更强。不同分类的Nesfatin-1水平之间预测RMR百分比存在微小显著差异。Nesfatin-1浓度较低的肥胖组的卡路里、碳水化合物和蛋白质摄入量也更高。
我们的数据表明脂肪百分比是循环Nesfatin-1水平的主要决定因素。看来Nesfatin-1和PPARγ可能同时参与脂肪生成途径。
