Brzhozovskiy A, Kononikhin A, Indeykina M, Pastushkova LKh, Popov I A, Nikolaev E N, Larina I M
1 Institute of Biomedical Problems - Russian Federation State Scientific Research Center, Russian Academy of Sciences, Moscow, Russia.
2 Moscow Institute of Physics and Technology, Moscow, Russia.
Eur J Mass Spectrom (Chichester). 2017 Aug;23(4):225-229. doi: 10.1177/1469066717717610. Epub 2017 Jul 5.
During the entire time that cosmonauts stay on board the international space station, different extreme space flight factors affect their bodies. In order to find out what physiological changes occur under the influence of spaceflight, different parameters of the human body before and after flights are monitored. Analysis of the urine proteome is one of the most perspective non-invasive methods of condition monitoring. The aim of the study was to perform a comparative semi-quantitative label-free urine proteome analysis of samples collected from 21 cosmonauts before and after long-duration spaceflight at the international space station. For proteomic analysis, urine samples were collected from cosmonauts at three time periods: six months prior to the flight as a background, and on days 1 and 7 of the recovery period after landing. All probes were analyzed by LC-MS/MS, and 256 proteins were identified with more than one unique peptide. The core proteome consists of 50 proteins that are detected in more than 70% of the samples. Label-free semi-quantitative analysis enables us to find 20 proteins which were significantly changed on +1 day and +7 day with respect to background. Most of these proteins participate in the regulation of biological processes, in the regulation of the immune system and in intracellular processes also; some of these proteins are related with stress and response to stimulus. In conclusion, the proteomic analysis of cosmonauts' urine samples provides new data on the human body's adaptation to ground conditions after long-duration spaceflight.
在宇航员驻留在国际空间站的整个期间，不同的极端太空飞行因素会影响他们的身体。为了弄清楚在太空飞行的影响下会发生哪些生理变化，飞行前后人体的不同参数都受到监测。尿液蛋白质组分析是最具前景的非侵入性健康状况监测方法之一。本研究的目的是对从21名宇航员在国际空间站进行长期太空飞行前后采集的样本进行比较性的半定量无标记尿液蛋白质组分析。为了进行蛋白质组分析，在三个时间段从宇航员身上采集尿液样本：飞行前六个月作为背景样本，以及着陆后恢复期的第1天和第7天。所有样本均通过液相色谱-串联质谱法（LC-MS/MS）进行分析，共鉴定出256种蛋白质且每种蛋白质都有不止一条独特肽段。核心蛋白质组由50种蛋白质组成，这些蛋白质在超过70%的样本中都能检测到。无标记半定量分析使我们能够找出20种在第1天和第7天相对于背景样本有显著变化的蛋白质。这些蛋白质中的大多数参与生物过程的调节、免疫系统的调节以及细胞内过程；其中一些蛋白质与应激和对刺激的反应有关。总之，对宇航员尿液样本的蛋白质组分析为长期太空飞行后人体对地面条件的适应提供了新的数据。
