一种用于检测哺乳动物组织匀浆中超氧化物歧化酶活性的测定方法。
An assay for superoxide dismutase activity in mammalian tissue homogenates.
作者信息
Spitz D R, Oberley L W
机构信息
Radiation Research Laboratory, University of Iowa, Iowa City 52242.
出版信息
Anal Biochem. 1989 May 15;179(1):8-18. doi: 10.1016/0003-2697(89)90192-9.
During the course of measuring superoxide dismutase (SOD) activity in rat breast tissue, interferences in the nitroblue tetrazolium (NBT) and cytochrome c assay systems were noted. These interferences inhibit accurate measurement of SOD activity in breast tissues, necessitating the development of a new NBT-based assay that includes compounds capable of inhibiting tissue specific interferences. The most effective compounds were metal chelators that were also electron transport chain inhibitors. Bathocuproine sulfonate (BCS) was the most effective of these compounds. The inclusion of BCS in the NBT assay system was shown to make the accurate measurement of SOD activity in tissues with interferences possible.
在测量大鼠乳腺组织中超氧化物歧化酶（SOD）活性的过程中，发现了对氮蓝四唑（NBT）和细胞色素c检测系统的干扰。这些干扰会抑制乳腺组织中SOD活性的准确测量，因此需要开发一种基于NBT的新检测方法，该方法要包含能够抑制组织特异性干扰的化合物。最有效的化合物是金属螯合剂，它们也是电子传递链抑制剂。磺酸铜试剂（BCS）是这些化合物中最有效的。结果表明，在NBT检测系统中加入BCS可以使在存在干扰的组织中准确测量SOD活性成为可能。
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