Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology (ECUST), 130 Meilong Rd., Shanghai 200237, PR China.
National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica (SIMM), Chinese Academy of Sciences (CAS), 189 Guo Shoujing Rd., Shanghai 201203, PR China.
Chem Soc Rev. 2015 Jul 7;44(13):4239-4248. doi: 10.1039/c4cs00252k. Epub 2014 Dec 4.
Construction of composite materials based on the self-assembly of fluorescently labeled biomolecules with a variety of micro- or nano-quenching materials (by the Förster Resonance Energy Transfer mechanism) for the fluorogenic recognition of disease-related proteins has become a dynamic research topic in the field of fluorescence recognition. Here we summarize the recent progress on the composition of fluorescence dye-labeled biomolecules including sugars, peptides and nucleotides with organic (graphene and carbon nanotubes) and inorganic (gold nanoparticles) materials. Their application in the fluorescence detection of proteins and enzymes on both the molecular and cellular levels is discussed. Perspectives are proposed with respect to the future directions of employing these composite materials in the recognition of pathological proteins.
基于荧光标记生物分子与各种微纳级猝灭材料（通过Förster 共振能量转移机制）的自组装构建用于疾病相关蛋白的荧光识别的复合材料已成为荧光识别领域中的一个活跃研究课题。在此，我们总结了荧光染料标记生物分子（包括糖、肽和核苷酸）与有机（石墨烯和碳纳米管）和无机（金纳米粒子）材料组成的最新进展。讨论了它们在分子和细胞水平上对蛋白质和酶的荧光检测中的应用。并就这些复合材料在病理蛋白识别中的应用的未来方向提出了展望。
