肽与氧化石墨烯的相互作用及其在蛋白酶活性实时监测中的应用。
Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity.
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong RD 130, Shanghai, 200237, China.
出版信息
Chem Commun (Camb). 2011 Feb 28;47(8):2399-401. doi: 10.1039/c0cc04887a. Epub 2010 Dec 16.
PMID:21305066
Abstract
We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.
摘要
我们开发了一种新型基于石墨烯的生物传感平台，使用肽作为探针生物分子，并基于 GO 与染料标记肽之间的 FRET，展示了其在实时监测蛋白酶活性方面的应用可行性。该测定法可快速准确地确定酶动力学参数和抑制常数。
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