首次从泰国北部分离出利什曼原虫：病例报告，鉴定为马提尼克利什曼原虫及其在恩氏利什曼原虫复合体中的系统发育位置

First isolation of Leishmania from Northern Thailand: case report, identification as Leishmania martiniquensis and phylogenetic position within the Leishmania enriettii complex.

作者信息

Pothirat Thatawan, Tantiworawit Adisak, Chaiwarith Romanee, Jariyapan Narissara, Wannasan Anchalee, Siriyasatien Padet, Supparatpinyo Khuanchai, Bates Michelle D, Kwakye-Nuako Godwin, Bates Paul A

机构信息

Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

出版信息

PLoS Negl Trop Dis. 2014 Dec 4;8(12):e3339. doi: 10.1371/journal.pntd.0003339. eCollection 2014 Dec.

Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes within macrophages. Isolation of Leishmania LSCM1 into culture and DNA sequence analysis (ribosomal RNA ITS-1 and large subunit of RNA polymerase II) revealed the parasites to be members of the Leishmania enriettii complex, and apparently identical to L. martiniquensis previously reported from the Caribbean island of Martinique. This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region. Moreover, the majority of parasites previously identified as "L. siamensis" also appear to be L. martiniquensis.

自1996年以来，泰国已有多例关于本地内脏利什曼病的病例报告。在此，我们报告一例来自泰国北部的52岁男性病例，该患者表现为亚急性发热、巨脾和全血细胞减少。骨髓穿刺显示巨噬细胞内有大量无鞭毛体。将利什曼原虫LSCM1分离培养并进行DNA序列分析（核糖体RNA ITS-1和RNA聚合酶II大亚基），结果显示这些寄生虫属于利什曼原虫恩氏复合体，且显然与先前在加勒比海马提尼克岛报道的马提尼克利什曼原虫相同。这是该地区首例由马提尼克利什曼原虫引起的内脏利什曼病报告。此外，大多数先前被鉴定为“暹罗利什曼原虫”的寄生虫似乎也是马提尼克利什曼原虫。