Bathgate B, Baker A, Leaver C J
Department of Botany, University of Edinburgh, Scotland.
Eur J Biochem. 1989 Aug 1;183(2):303-10. doi: 10.1111/j.1432-1033.1989.tb14929.x.
Southern blot hybridisation of maize (Zea mays) genomic DNA with a specific cDNA probe and sequence analysis of several cDNAs have revealed that the mitochondrial adenine nucleotide translocator (ADP/ATP carrier) is encoded by two genes, both of which are expressed in dark-grown coleoptile tissue. The structural genes (designated ANT-G1 and ANT-G2), encoding the two translocator polypeptides, have been isolated from a maize genomic library and characterised by DNA sequence analysis, mung bean nuclease mapping and primer extension. The two genes each contain two introns and encode very similar proteins. Transcripts from both genes have long (approximately 200 nucleotides) 5' untranslated leaders which are unusual in that they contain several ATG codons upstream of the initiation codon. Transcript analysis of RNA extracted from serial sections of developing maize leaves reveals that the translocator genes are most actively transcribed in the basal meristem, while transcripts were undetectable in green leaves.
用特定的cDNA探针与玉米（Zea mays）基因组DNA进行Southern杂交，以及对几个cDNA进行序列分析，结果表明线粒体腺嘌呤核苷酸转运体（ADP/ATP载体）由两个基因编码，这两个基因在黑暗生长的胚芽鞘组织中均有表达。编码两种转运体多肽的结构基因（命名为ANT-G1和ANT-G2）已从玉米基因组文库中分离出来，并通过DNA序列分析、绿豆核酸酶作图和引物延伸进行了表征。这两个基因各包含两个内含子，编码非常相似的蛋白质。来自这两个基因的转录本都有很长（约200个核苷酸）的5'非翻译前导序列，其不同寻常之处在于它们在起始密码子上游包含几个ATG密码子。对从发育中的玉米叶片连续切片中提取的RNA进行转录本分析表明，转运体基因在基部分生组织中转录最活跃，而在绿叶中未检测到转录本。
