玉米ATP/ADP转运体cDNA的分离与序列分析
Isolation and sequence analysis of a cDNA encoding the ATP/ADP translocator of Zea mays L.
作者信息
Baker A, Leaver C J
出版信息
Nucleic Acids Res. 1985 Aug 26;13(16):5857-67. doi: 10.1093/nar/13.16.5857.
Abstract
A cDNA complementary to the mRNA for the ATP/ADP translocator of maize (Zea mays L.) has been identified by virtue of hybridisation with the homologous gene from yeast. The cloned cDNA has been shown by DNA sequence analysis to contain an open reading frame of 954bp., which encodes a polypeptide of molecular weight 40,519. This polypeptide exhibits a high degree of homology to the translocator polypeptides of beef heart and Neurospora crassa mitochondria.
摘要
通过与酵母中的同源基因杂交，已鉴定出与玉米（Zea mays L.）ATP/ADP转位酶mRNA互补的cDNA。DNA序列分析表明，克隆的cDNA含有一个954bp的开放阅读框，编码分子量为40,519的多肽。该多肽与牛心和粗糙脉孢菌线粒体的转位酶多肽具有高度同源性。
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