人参皂苷Rg1和Rb1与丹酚酸B联合使用，在大鼠心肌梗死中发挥不同作用。
Ginsenoside Rg1 and Rb1, in combination with salvianolic acid B, play different roles in myocardial infarction in rats.
作者信息
Deng Yanping, Zhang Tingting, Teng Fukang, Li Defang, Xu Feng, Cho Kenka, Xu Jinghua, Yin Jun, Zhang Li, Liu Qian, Yang Ming, Wu Wanying, Liu Xuan, Guo De-An, Jiang Baohong
机构信息
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; Shenyang Pharmaceutical University, Shenyang, China.
出版信息
J Chin Med Assoc. 2015 Feb;78(2):114-20. doi: 10.1016/j.jcma.2014.10.001. Epub 2014 Dec 1.
BACKGROUND
The herb pair of Salvia miltiorrhiza and Panax notoginseng has widely been used for improving coronary and cerebral circulation in China. However, the exact contribution of the major active components of S. miltiorrhiza and P. notoginseng to cardioprotection is far from clear. In the present study, three representative ingredients, salvianolic acid B (SalB) from S. miltiorrhiza and ginsenoside Rg1 (Rg1) and ginsenoside Rb1 (Rb1) from P. notoginseng, were selected to elucidate the mechanism of the herb pair at the ingredient level.
METHODS
The purity of SalB, Rg1, and Rb1 was >99%, as detected by high-performance liquid chromatography. Acute myocardial infarction was introduced by ligation of the left anterior descending coronary artery near the main pulmonary artery. Cardiac contractility was detected through a Mikro-tipped catheter, and cardiac infarct size was determined using triphenyltetrazolium chloride stain.
RESULTS
The combination of SalB and Rg1, and not the combination of SalB and Rb1, improved heart contractility in rats with myocardial infarction. The different contributions of Rg1 and Rb1, in combination with SalB, to cardioprotection provides further direction to optimize and modernize the herbal medicines containing S. miltiorrhiza and P. notoginseng.
CONCLUSION
The combination of SalB and Rg1 may provide potential protection against myocardial infarction.
背景
丹参和三七这味药对在中国已被广泛用于改善冠脉循环和脑循环。然而，丹参和三七主要活性成分对心脏保护的确切作用尚不清楚。在本研究中，选取了三种代表性成分，即丹参中的丹酚酸B（SalB）以及三七中的人参皂苷Rg1（Rg1）和人参皂苷Rb1（Rb1），以在成分层面阐明该药对的作用机制。
方法
通过高效液相色谱法检测，SalB、Rg1和Rb1的纯度均>99%。通过结扎主肺动脉附近的左前降支冠状动脉诱导急性心肌梗死。通过微尖端导管检测心脏收缩力，并使用氯化三苯基四氮唑染色法测定心脏梗死面积。
结果
SalB与Rg1的组合而非SalB与Rb1的组合改善了心肌梗死大鼠的心脏收缩力。Rg1和Rb1与SalB联合时对心脏保护的不同作用为优化和现代化含丹参和三七的草药提供了进一步的方向。
结论
SalB与Rg1的组合可能对心肌梗死具有潜在的保护作用。
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