Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.
Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.
Talanta. 2015 Jan;132:215-21. doi: 10.1016/j.talanta.2014.09.010. Epub 2014 Sep 16.
A unique nanocomposite was crafted by grafting hemin-binding-aptamer (HBA) onto carboxylated graphene (COO-GR). Infrared spectroscopy, Raman spectroscopy and diffuse reflectance spectra suggested that -NHCO- covalent bonds were formed between HBA and COO-GR. The resulting COO-GR/HBA functionalized electrode was used as a novel label-free biosensor. The square wave voltammetry was employed to realize the selective and specific detection of hemin. The obtained aptasensor possessed excellent performance with a detection limit of 0.64 nmol L(-1) (S/N=3) and a linear range from 1 to 150 nmol L(-1). Moreover, COO-GR was shown to be a promising candidate in making aptasensors, carrying advantages over graphene in terms of the simplicity of sensor preparation and the reduction of background noise.
通过将血红素结合适体（HBA）接枝到羧基化石墨烯（COO-GR）上，制备了一种独特的纳米复合材料。红外光谱、拉曼光谱和漫反射光谱表明，HBA 和 COO-GR 之间形成了-NHCO-共价键。将得到的 COO-GR/HBA 功能化电极用作新型无标记生物传感器。采用方波伏安法实现了对血红素的选择性和特异性检测。所得适体传感器具有优异的性能，检测限为 0.64 nmol L(-1)（S/N=3），线性范围为 1 至 150 nmol L(-1)。此外，COO-GR 被证明是一种很有前途的适体传感器制备材料，与石墨烯相比，其具有传感器制备简单和降低背景噪声的优点。
