College of Resources and Environment, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2012 Jun-Jul;36(1):186-91. doi: 10.1016/j.bios.2012.04.011. Epub 2012 Apr 17.
A novel label-free electrochemical aptasensor based on graphene-polyaniline (GR-PANI) nanocomposites film for dopamine (DA) determination was reported. The resulting GR-PANI layer exhibited good current response for DA determination. The good electron transfer activity might be attributed to the effect of GR and PANI. The highly conductive and biocompatible nanostructure of GR-PANI nanocomposites was characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). To quantify the amount of DA, the peaks of square-wave voltammetry (SWV) were monitored using the redox couple of an Fe(CN)(6) probe. The electrochemical aptasensor showed a linear response to DA in the range 0.007-90 nmol/L and a limit of detection of 0.00198 nmol/L (S/N=3). The electrochemical aptasensor was successfully tested on human serum samples.
基于石墨烯-聚苯胺（GR-PANI）纳米复合材料薄膜的新型无标记电化学适体传感器用于多巴胺（DA）的测定。所得的 GR-PANI 层对 DA 的测定表现出良好的电流响应。良好的电子转移活性可能归因于 GR 和 PANI 的作用。GR-PANI 纳米复合材料的高导电性和生物相容性纳米结构通过扫描电子显微镜（SEM）、循环伏安法（CV）和电化学阻抗谱（EIS）进行了表征。为了定量测定 DA 的量，使用 Fe(CN)(6)探针的氧化还原对监测方波伏安法（SWV）的峰。电化学适体传感器对 0.007-90 nmol/L 范围内的 DA 表现出线性响应，检测限为 0.00198 nmol/L（S/N=3）。该电化学适体传感器已成功应用于人血清样品的测试。
